【作者】 王波；

【导师】 任国平；

【作者基本信息】 浙江大学 ， 病理学与病理生理学， 2006， 硕士

【摘要】 目的:观察RON及其变异体在肺腺癌中的表达,探讨RON及其变异体的表达与肺癌的组织学类型及组织学分级之间的相关性。 方法:收集2003.1～2005.12在我院病理科存档的肺腺癌石蜡组织标本106例,肺鳞癌石蜡标本10例,应用Envision二步法检测106例肺腺癌及10例肺鳞癌中RON的表达情况,根据染色强度将结果分为四个等级,即阴性、弱阳性、中等阳性和强阳性,并结合临床资料进行统计学分析;收集2004.1～2005.12在我院的冰冻肺癌标本46例,其中包括腺癌37例,鳞癌9例,通过Western-Blot检测肺癌组织中RON及其变异体的表达情况,并对RON在不同组织学分级和不同组织学类型中的表达进行统计学分析。 结果:RON的阳性表达定位于肿瘤细胞胞浆及胞膜上。RON在肺腺癌及鳞癌中都有不同程度的表达,两组相比RON的表达具有显著性差异(p<0.05);不同分化程度的肺腺癌相比,RON的表达也有差异,低分化的肺腺癌明显高于中分化的腺癌和高分化的腺癌(p<0.05);Western-Blot结果显示在肺腺癌和肺鳞癌中均有RON表达;在肺腺癌中观察到RON的变异体,而肺鳞癌中则没有。更多还原

【Abstract】 Obejective To investigate the expression of RON and its variants in human lung adenocarcinomas. Explore the relationship between expression of RON and the differentiated grade of human lung cancers.Methods A total of 106 paraffin-embedded human lung adenocarcinomas and 10 human lung squamous carcinomas from 2003 to 2005 were obtained from the Department of Pathology in the First Affiliated Hospital of Zhejiang University. The expression of RON was detected by immunohistochemistry. The levels of RON expression were assessed using the average staining intensity. Statistical analysis were done. Frozen lung cancers(37 human lung adenocarcinomas and 9 human lung squamous carcinomas) were also obtained from the Department of Pathology in the First Affiliated Hospital of Zhejiang University. Expression of RON and its variants were detected by Western-blot.Results The positive staining was located in the cytoplasm and cell surface of the tumor cells.RON gene was lowly expressed in human lung squamous carcinomas. High level expression was seen in human lung adenocarcinomas(p<0. 05). Compared with the well-differentiated and moderate-differentiated adenocarcinomas, RON expression in the low-differentiated ones significantly increased(p<0.05).RONvariants were found in human lung adenocarcinomas by Western-blot but not in squamous carcinomas.Conclusions RON express in human lung adenocarcinomas and squamous carcinomas, which are correlated with the tumor type and clinical stage.RON variants can also express in human lung adenocarcinomas but not in squamous carcinomas.更多还原