【作者】 王大力；

【导师】 金东日；

【作者基本信息】 延边大学 ， 分析化学， 2005， 硕士

【摘要】 槲皮素、山柰酚、异鼠李素等及其衍生物是广泛存在于各种蔬菜和水果中的黄酮类化合物。本论文选择了槲皮素、山柰酚、杨梅黄酮及桑色素四种结构类似的黄酮类化合物,应用HPLC分析方法结合质谱检测技术,确定酸水解样品中是否存在四种黄酮苷元,利用质谱及文献中紫外光谱、质谱数据确定可能存在的其它黄酮苷元。在此基础上分析样品中黄酮类化合物原型成分,建立一种适用于实际样品中几种黄酮类化合物的HPLC分析方法。 应用上述HPLC法分析了洋葱中的黄酮类化合物,同时使用半制备柱采用高效液相色谱法分离制备了洋葱中两种主要的黄酮类化合物。 具体研究内容如下: 1、建立了几种黄酮类化合物的HPLC分析方法。 2、利用HPLC法结合质谱定性、定量分析洋葱中黄酮苷元。 应用正交实验确定了洋葱的最佳水解条件,测定了二批洋葱中槲皮素的含量。 3、采用二极管阵列检测(DAD)和质谱(MS)检测技术与HPLC联用分析洋葱中黄酮类化合物结构或可能结构。 4、首次利用半制备柱采用高效液相色谱法分离制备了洋葱中两种主要的黄酮类化合物(纯度超过98%)。应用UV、MS、IR、NMR确定其结构。其结构为3,5,7,3′,4′-五羟基黄酮(quercetin)和槲皮苷(quercetin-4′-glucoside)。更多还原

【Abstract】 In vegetables and fruits,quercetin,kaempferol,isorhamntin et and their derivations are widely presentln this dissertation we have selected four flavonoids(quercetin,kaempferol, myricetin and morin) which have similar structural flavonoid compounds.The analysis method of HPLC-MS was applied to determine whether flavonoid aglycones consisted in the sample or not.It may also be applied to the determination of other flavonoid aglycones by their UV spectral and mass spectrometric date,and by comparison with literature date.On the basis of this, archetypal flavonoid compounds were determinated.And then we have developed a method for the determination of several flavonoids in practical samples.Applying above-mentioned HPLC to analyse flavonoids in the onion,and two flavonoids in the onion were isolated and prepared by semi-preparative HPLC,1 .Establish the method of analyse several flavonoid compounds by HPLC.2. HPLC and HPLC-MS are applied to the quantification and qualification determination of flavonoid aglycones in the onion.The proper hydrolysis condition was given by orthogonal experimentAnd determine the content of quercetin in two group onion.3. Analyse the structure of flavonoid compounds and possible structure by HPLC/D AD/MS.4. Two main flavonoids in the onion were isolated and prepared by semi-preparative HPLC at the first time(purity more than 98%). And their structure were elucidated through UV,IR,MS,NMR techniques.One was identified as 3,5,7,3’,4’-pentahydraoxy-flavone(namely quercetin),and the other one was identified quercetin-4’-glucoside.更多还原