【作者】 胡琳琳；

【导师】 高云芳；

【作者基本信息】 西北大学 ， 中药学， 2005， 硕士

【摘要】 自1980年北京师范大学生物系首次用大黄复制脾虚证动物模型以来,已有十多种动物模型相继建立。动物模型的建立,使关于脾虚证较深入的实验研究成为可能。目前研究者已对各种脾虚证动物在消化吸收、免疫、神经、内分泌等方面的改变进行了探讨。但是,实验研究中常用的脾虚证动物模型与临床脾虚证还存在一定的差距。传统中医认为“劳”作为病因包括劳力过度、劳神过度和房劳过度三方面,而现有的“劳倦过度”脾虚证动物模型,主要采用力竭运动造成动物身体疲劳,对动物精神的影响不明显。所谓“劳倦过度”造模方法仅模拟了劳力过度,没有模拟劳神过度这一因素,与临床脾虚证有一定差距。另外,多数脾虚证造模方法为单因素造模,仅有少数几种复合因素脾虚证模型。因此,对现有“劳倦过度”脾虚证单因素动物模型进行改善,建立一个比较接近临床脾虚证的动物模型已成为脾虚证本质研究的迫切需求。 为了更好地模拟劳力过度和劳神过度两方面的致病原因,本实验在以往力竭游泳单因素塑造脾虚证动物模型的基础上剥夺动物的部分睡眠时间,使动物不仅劳力而且劳神。从而建立了一种新的脾虚证动物模型——改良型劳倦过度小鼠脾虚证模型。 实验主要观察了动物的一般体征和消化吸收功能的变化,测定的指标有:D—木糖排泄率、血清淀粉酶活性、胃排空速率、肠推进速率、离体小肠张力、胃肠粘膜形态、血清胃泌素含量、脾脏指数和胸腺指数。通过与大黄泻下法、力竭游泳法塑造的脾虚证动物模型比较、药物反证等几方面来评估和验证此方法的成立,并对其影响动物消化系统功能的机制作了初步探讨。 实验结果显示,改良型劳倦过度模型小鼠造模后出现脾虚证的典型外观表现,且客观指标改变与文献报导相符,与临床脾虚证患者变化趋势一致。在所选的指标中,改良型劳倦过度组小鼠的变化均与临床脾虚证患者的变化趋势一致,且多数指标的变化较大黄组(大黄泻下法塑造脾虚证动物模型)和力竭游泳组(力竭游泳法塑造脾虚证动物模型)明显,而大黄组和力竭游泳组只有部分指标的变化与临床患者一致,因此,改良型劳倦过度法塑造脾虚证动物模型优于大黄泻下法和力竭游泳法。运用四君子汤治疗进行反证,小鼠一般体征、各客观指标较自然恢复组明显改善,说明改良型劳倦过度法塑造的脾虚证模型符合脾虚证动物模更多还原

【Abstract】 Since Beijing Normal University established rhubarb laxation spleen-asthenia Syndrome (SAS) animal model in 1980, more than ten animal models have been established. With the establishment of these animal models, it is possible to launch the further research of SAS. Now researchers have discussed symptoms of SAS animals in digestion and absorption, immunity, nerve and endocrine etc. However, SAS animal models in lab are much different from clinical SAS. According to traditional Chinese medical theory, as cause of illness, over-fatigue includes overwork, restlessness, and over- coitus. But current fatigue SAS model makes animals fatigued by over-exercise. And it has little influence on spirit of animals. Fatigue model only simulates overwork, but not restlessness. Therefore, it is quite different from clinical SAS. On the other hand, most SAS models only consider single factor. Only a few models include several factors. Accordingly, it is greatly necessary to improve current fatigue SAS model with single factor, and establish a new SAS model that similar to clinical SAS to study the essence of SAS.In order to simulate illness causes including overwork and restlessness, we make animals over-swimming, as is done in normal SAS models, and partially deprive animals of their rest time at the same time. Animals are not only tired but also restless. Based on this, we established an improved SAS animal model, namely improved over-fatigue mice SAS model.The change of general physiological characters, D-xylose excretion, activity of amylase in serum, stomach emptying and enterokinesia speed, contractile activity of small intestine in vitro, morphological change of gastrointestinal mucosa, gastrin (Gas) content in serum, spleen index, thymus index were observed. In order to evaluate our improved over-fatigue model, we compare it with rhubarb laxation SAS model and over-swimming SAS model, and proved by Sijunzi decoction. Moreover, we elementarily studied the mechanism of the changes in digestion and absorption function by improve over-fatigue method.The results showed improved over-fatigue mice represented typical SAS.Moreover, changes in objective indexes are consistent with literature. These changes have a coherent trend to those of SAS clinical patents, and most changes were more remarkable than the rhubarb root laxation and over-swimming SAS mice models. But only partial changes of rhubarb laxation group and over-swimming group have a coherent trend to those of SAS clinical patents. Our improved over-fatigue method is better than rhubarb laxation method and over-swimming method. Proved by Sijunzi decoction treatment, the mice behave normally. All objective indexes are improved more distinctively than the group that resumes naturally. These prove that the SAS model is made successfully and meets the requirements of judgement for SAS animal model. In this model, SAS is caused possibly by largely released Gas, which results in increased contractile in small intestine, over-exudation of intestine and obstacle in absorption. These changes are also basic pathologies of SAS symptoms.As a new SAS animal model, it simulates several SAS causes, approaches the pathologic changes of clinical patients. It is a more appropriate model to study the essence of SAS.更多还原