【作者】 高翔；

【导师】 张立实； 包大跃； 计融；

【作者基本信息】 四川大学 ， 营养与食品卫生学， 2005， 硕士

【摘要】 本课题利用⁶⁰Coγ射线在常温下对生理盐水及粮食（小麦、玉米）中不同浓度的黄曲霉、赭曲霉、串珠镰刀菌孢子以及猪肉中的囊尾蚴进行了辐照杀灭研究。结果表明:（1）⁶⁰COγ射线对上述三种霉菌孢子及囊尾蚴有明显的致死作用,并有良好的计量—反应关系;（2）浓度相近的霉菌孢子在生理盐水中的D₁₀值低于在粮食中的D₁₀值;（3）在相同介质中,低浓度霉菌孢子的D₁₀值高于高浓度霉菌孢子的D₁₀值;（4）三种霉菌的抗辐射性大小依次为串珠镰刀菌、赭曲霉、黄曲霉;（5）在实验设计的孢子浓度下,黄曲霉孢子在生理盐水中的D₁₀值为0.39～0.47kGy,最小杀灭剂量为1.5～2.5kGy,在玉米中的D₁₀值为0.67～0.72kGy,最小杀灭剂量为2～3kGy。赭曲霉孢子在生理盐水中的D₁₀值为0.46～0.51kGy,最小杀灭剂量为1.5～3.0kGy,在小麦中的D₁₀值为0.60～0.66kGy,最小杀灭剂量为2.0～3.0kGy。串珠镰刀菌孢子在生理盐水中的D₁₀值为0.57～0.60kGy,最小杀灭剂量为3.0～4.0kGy,在小麦中的D₁₀值为0.66～0.81kGy,最小杀灭剂量为3.0～4.0kGy。猪肉中囊尾蚴的最小辐照杀灭剂量为4.5kGy。综合以上结果,杀灭自然条件下粮食中上述霉菌孢子的辐照剂量应为3.0～4.0kGy,杀灭猪肉中囊尾蚴的辐照剂量应为4.5kGy。更多还原

【Abstract】 The effects of ⁶⁰Co gamma irradiation at different dosage on Aspergillus flavus spores, Aspergillus ochraceus spores and Fusarium momiliiforme spores in both wheat or corn and physiologic saline solution, and cysticerci in pork were studied. The results showed that （1） ⁶⁰Co Gamma-irradiation has significantly lethal effect on these three fungal spores and cysticerci. （2）The irradiation D₁₀ values of fungal spores in physiologic saline solution were lower than that in cereals. （3） High concentration of fungal spores has a lower D₁₀ value in the same medium than the low concentration. （4）An increase in radioresistance is in the following order: Aspergillus flavus, Aspergillus ochraceus and Fusarium momiliiforme. （5）The D₁₀ values of Aspergillus flavus spores in physiologic saline solution and in corns ranged from 0.39kGy to 0.47kGy and 0.67kGy to 0.72kGy, respectively. The dose required for complete inhibition of Aspergillus flavus spores in physiologic saline solution and in corns ranged from 1.5kGy to 2.5kGy and 2kGy to 3kGy, respectively. The D₁₀ values of Aspergillus ochraceus spores in physiologic saline solution and in corns ranged from 0.46kGy to 0.51kGy and 0.60kGy to 0.66kGy, respectively. The dose required for complete inhibition of Aspergillus ochraceus spores in physiologic saline solution and in corns ranged from 1.5kGy to 3.0kGy and 2.0kGy to 3.0kGy, respectively. Thevalues of Fusarium momiliiforme spores in physiologic saline solution and in corns ranged from 0.57kGy to 0.60kGy and 0.66kGy to 0.81kGy, respectively. The dose required for complete inhibition of Aspergillus ochraceus spores in physiologic saline solution and in corns ranged from 3.0kGy to 4.0kGy and 3.0kGy to 4.0kGy, respectively. The dose required for killing cysticerci in pork was 4.5kGy. According to the results of our study, it is concluded that the suggested dose required for killing Aspergillus flavus spores, Aspergillus ochraceus spores and Fusarium momiliiforme spores under natural conditions ranged from 3.0kGy to 4.0kGy and 4.5kGy for cysticerci.更多还原