【作者】 刘道德；

【导师】 王凡；

【作者基本信息】 四川大学 ， 人体解剖与组织胚胎学， 2004， 硕士

【摘要】 目的:中药在骨折的治疗上有悠久的历史,并取得了一定的成效。但由于对骨折愈合机制及药物作用机理不清楚,故未取得较大的突破。本课题通过对中药淫羊藿对家兔骨折愈合的作用及作用机制的探讨,为淫羊藿促进骨折愈合的临床应用提供理论基础,同时也为中药对骨折愈合的促进作用的深入研究积累一些经验。 方法:水合氯醛腹腔麻醉下,手术制作成年雄性家兔双前肢桡骨中段骨折动物模型（缺损约 3mm）。术后随机将其分为两组,分笼喂养,自由活动及进食,实验组除每日给予淫羊藿煎液口服（6mg/kg/d）外,其他饲养条件完全与对照组相同。分别于术后14天、24天、34天及42天处死动物,处死前摄兔前肢X光片,处死后取骨折标本作连续硬组织切片（厚5um）,切片作常规甲苯胺蓝染色及Masson三色染色,并作TGF-β₁及VEGF免疫组化染色。 结果:1、淫羊藿实验组各阶段X光片显示骨折的骨痂生更多还原

【Abstract】 Objective Traditional Chinese medicine has been used to treat fracture since long ago. Though to certain extent the effect has been achieved, little breakthrough has been made for the mechanism for fracture healing and the way the medicine works remain yet unknown. This study bears the objective of exploring the mode of action of Epimedium as it help the healing of the fracture in rabbit, and thus, provide theoretic foundation for its clinical application. It serves to provide reference for more in-depth studies of this field.Methods Matured, male rabbits were anesthetized by injection of chloral hydrate into their abdominal cavity. Both radius of the rabbits were surgically fractured （damaged area app. 3mm）. The rabbits were randomly divided into experimental group and control group, housed separately. They were free to move and feed. The experimental group have all other theEpimedium extract （6mg/kg/d） orally. Termination of the animals took place in day 14,day 24, day 34 and day 42 respectively, after taking the X-ray of their forelimb. 5um section were made from the fractured sclerous tissue. The samples were dyed with routine toluidine blue, Masson dye and immunohistochemistry of TGF-β₁ and VEGF.Results 1. Radiograph taken in every period showed that growth of callus 、 bony remodeling and recanalization of medullary cavity is more prominent in the experimental group.2. Dyeing of toluidine blue showed that the formation of cartilage and osteoclast、 endochondral bone formation and intramembranous ossification、 the formation and remodeling of callus and recanalization of medullary cavity of experimental group are earlier than that of control group.3. Masson dyeing showed that quantity and density of callus of experimental group are greater than control group’s, the formation of connecting callus 、 remodeling of callus andrecanalization of medullary cavity of experimental group were earlier than control group’s.4. Immunohistochemical staining of TGF-j3i and VEGF of experimental group is more denser than control group up to the 24th day, the difference is insignificant from then onwards.Conclusion Epimedium can promote fracture healing of rabbits by enhancing expression of TGF-Pj and VEGF, then promoting regeneration of vascellum, promoting collection^ proliferation and differentiation of mesenchymal cell , proliferation and differentiation of osteoblast and chondrocyte ^ advancing formation of osteoclast, promoting formation and remodeling of callus and recanalization of medullary cavity.更多还原