【作者】 沈颉飞；

【导师】 杜莉；

【作者基本信息】 四川大学 ， 口腔临床医学， 2004， 硕士

【摘要】 磁体在口腔临床的运用越来越广泛,其中磁性附着体作为新型的义齿固位力来源在口腔修复学领域显示了其独特的优势。在磁性附着体从早期开放磁路系统到现今闭合磁路系统的设计发展历程中,各种磁体所产生的静磁场是否具有口腔生物学安全性一直存在不同见解,其中又以磁场对牙周组织的生物学效应为讨论的热点。 本实验使用四川大学华西口腔医学院修复科自主研制的磁场加载系统,对体外培养的人牙龈成纤维细胞进行加载,检测磁场对细胞碱性磷酸酶和超氧化物歧化酶活性的影响。通过磁场加载系统所产生的120mT及10mT静磁场,分别模拟开放磁路系统和闭合磁路系统所产生的磁场作用,对人牙龈成纤维细胞进行以24小时为一周期的循环间断加载。使用全自动生化分析仪以及超氧更多还原

【Abstract】 The application of oral magnets has been broaden increasingly in recent years, especially for the magnetic attachments. As a new type of sources for denture retentive force, the magnetic attachments have shown their unique advantages in the domain of prosthetic dentistry. In their course of development from early open-circuit magnet system to nowadays close-circuit magnet system, for different type of magnets, controversial opinions persist about whether their static magnetic fields have oral biosecurity. Among those opinions, more emphasis has been paid to the effects of magnets on the periodontal tissues.In this study, a magnet loading system which was developed by the department of prosthetic dentistry in the West Chine college of stomatology, Sichuan university, was used to impose the static magnetic force on the human gingival fibroblasts (HGF) in vitro to investigate if there was any activity change of alkaline phosphatase (ALP) and superoxide dismutase (SOD) in those cells. The 120mTand lOmT magnetic fields generated by the system were designed to stimulate the effects of open-circuit and close-circuit magnetic attachments on the gingiva respectively. For each amount of magnetic fields intensity, 3 groups of repeated loading process with intervals were carried out with different numbers of 24-hour periodic cycles. And automatic biochemical analyzer and a SOD test box were applied to measure the activity of ALP and SOD accordingly. Comparison between the test and control groups that differ in their magnetic intensity and loading time was also made to analyze the biological effects of the static magnetic fields. The results show as following:1. No significant difference (P>0.05) of ALP or SOD activity was found between all the groups when HGF were loaded with different intensity of magnetic fields for the same period of time;2. Nor any significant difference (P>0.05 ) of ALP or SOD activity was found between all the groups when HGF were loaded with the same intensity of magnetic fields for different periods of time.As far as the conditions in this study are concerned, the results above supported that with reference to the ALP and SOD in HGF, biosecurity cloud be announced for the static magnetic fields of the open-circuit magnetic attachments as well as the close-circuit ones.更多还原