【作者】 杨华；

【导师】 吴文君；

【作者基本信息】 西北农林科技大学 ， 农药学， 2005， 硕士

【摘要】 苦皮藤（ C e l a s t r u s a n g u l a t u s ） 是卫矛科南蛇藤属的一种多年生灌木,广泛分布于我国长江和黄河流域的丘陵浅山区,80 年代以来,我国学者对其化学成分进行了研究,表明该植物的有效成分为以α二氢沉香呋喃为母体结构的多元醇酯类化合物。本论文在973 项目的资助下,通过大孔吸附树脂柱层析和HPLC 制备等技术,结合生物活性追踪,对苦皮藤提取物中极性较弱的部分进行了分离, 以期分离出更多的同类化合物为进一步的三维定量构效关系研究（3D-OSAR） 及指纹图谱的研究作前期准备。主要结果如下: 1 分离得到13 个纯化合物,采用1HNMR,1CNMR 等现代波谱学方法进行了结构鉴定。经文献检索,其中YH-2,YH-10,YH-15,YH-17 为新化合物, 依次命名为1α,2α,8β-三乙酰氧基-9α-苯甲酰氧基-13-异丁酰氧基-4β,6p-二羟基-α-二氢沉香呋喃;1α,2α,13-三乙酰氧基-8α,9α-二呋喃甲酰氧基-4α,6pα二羟基-p-二氢沉香呋喃;1α,2α,6β,13-四乙酰氧基-8α-异丁酰氧基-9D-呋喃甲酰氧基-4β-羟基-β-二氢沉香呋哺;1α,2α,6β,8α-四乙酰氧基-13-异丁酰氧基-9β-9 呋哺甲酰氧基-4β-羟基-β-二氢沉香呋哺。YH-20 和YH-24 结构尚待最后鉴定,其余化合物均为已知化合物。2 生测结果表明:YH-2,YH-15,YH-1-8,YH-19,YH-21,YH-24 表现为毒杀活性,YH-24 和YH-2 的胃毒活性最高,LD50 分别是201.54 μg·g^-1,280.42 μg·g^-1,稍高于苦皮藤素V;YH-19 与苦皮藤素V 的活性相当,LD50 是307.13 μg·g^-1;YH-21 的活性低于苦皮藤素V,LD50 分别是410.04μg·g^-1;YHl5 的活性最差,LD50 是1656.40μg·g^-1。YH-17 和YH-20 则表现为麻醉活性,其中YH-20 的麻醉活性显著的高于YH-17,在浓度为1%时,8h 的麻醉率达到100 %。更多还原

【Abstract】 The Chinese bittersweet , Celastrus angulatus Max , is a traditional insecticidal plant ,widely distributed over the basin of Yellow River and Yangtze river . More than 60 P -dihydroagarofuan sesquiterpere polyolesters have been isolated since 1980 . In order to establish fingerprint and further study on 3D-QSAR . The studies on the further isolation of insecticidal component from Celastrus angulatus Max. The main results are as follows. 1 Thirteen compounds were isolated and their structures were elucidated by spectroscopic methods including ’H-NMR and 13C-NMR. Four of them ,YH-2 1 α,2 a ,8 β-triacetoxy-9α-benzoyloxy-13-isobutyryloxy-4 J3 ,6 β-dihydroxy-{3 -dihydroagarofuan,YH-101α,2α13-triacetoxy-8 α,9 β-difuroyloxy-4β,6β-dihydroxy-β-dihydroagarofuan,YH-15 1 α,2α,6β,13-tetraacetoxy-8α-isobutyryloxy-9β-furoyloxy-4β-hydroxy-β-dihydroagarofuan and YH-17 1α,2α,6 β,8α-tetraacetoxy-13-isobutyryloxy-9 β-furoyloxy-4 β-hydroxy-β-dihydroagarofuan are new componends. 2 The bioassay results against Mythimna separata Walker showed that YH-2, YH -15, Yh-18, YH-19,YH-21,YH-24 exhibited insecticidal activity. YH-2 (LD₅₀= 280.42 u g·g^-1) and YH-24(LD50=201.54 μg ·g^-1) exhibited stronger insecticidal activity than Celangulin V(LD50=300.99 μg ·g^-1);YH-19(LD5o=307.13μg ·g^-1)and Celangulin V represented the same insecticidal activity; YH-21 (LD50=410.04 μg ·g^-1 ) were weaker than Celangulin V. YH-17 and YH-20 exhibit narcotic action, narcotic action of YH-20 was stronger than YH-17. Narcotic rates of YH-20 was up to 100% at 8h, at the concentration of 1%.更多还原