【作者】 吴岳恒；

【导师】 汤顺清；

【作者基本信息】 暨南大学 ， 生物医学工程， 2005， 硕士

【摘要】 本实验采用粉末烧结法用不同的烧制温度制备不同原料配方的多孔玻璃陶瓷（BGC）,并通过扫描电镜（SEM）,X射线粉末衍射仪（XRD）,降解实验,溶血实验,细胞相容性实验等方法表征和选出降解适中,生物相容性优异的组织工程支架材料。XRD检测出BGC的主晶相为B-Ca（PO₃）₂。降解实验表明,钙磷摩尔比（Ca/P）小于0.50,7d,600℃烧制BGC的降解率可达8%;Ca/P大于0.50,降解率约2%。溶血实验表明Ca/P从0.51至0.43的BGC有较好的溶血性能。细胞实验表明烧制温度高于600℃的BGC有较好的细胞相容性能。 所选BGC的Ca/P为0.47,烧制温度680℃。通过降解实验和生物学实验检验所选BGC的降解能力和生物相容性能。对所选BGC进行66h浸泡实验,测其pH值,并用ICP考察其离子浓度。结果显示,BGC的体外降解过程分为两阶段,0-6h比6-66h的降解速度快约10倍。对所选BGC进行28d浸泡实验,测其失重率,并用XRD,SEM考察晶形改变及表面形貌。结果显示,28d浸泡失重率9.2%,XRD图谱说明磷酸铝晶体和焦磷酸镁晶体的在此玻璃陶瓷中的溶解度大于β-Ca（PO₃）₂晶体。生物学实验表明,此BGC有非常好的生物相容性能。 综上所述,可以预料此材料是一种性能优异的组织工程支架材料。更多还原

【Abstract】 In this paper, some kinds of porous calcium phosphate bioglass ceramics (BGC) were sintered at different temperature by calcium phosphate bioglass powder with different compositions. The BGCs were selected as a kind of tissue engineering materials by results of X-ray diffraction (XRD), scanning electron microscopy (SEM), degradations and biological compatibility experiments. β-Ca(PO3)2 phases are detected by X-ray diffraction in all kinds of porous BGCs. Results of the degradations demonstrate the degradation rates of porous BGCs are almost 8% as the Ca/P ratio less than 0.50,and the degradation rates are nearly 2% as the Ca/P ratio of porous BGCs more than 0.50. The hemolytic experiments show there are excellent properties as the Ca/P ratio of porous BGCs is from 0.51 to 0.43. The cytocompatibility experiments indicate that the BGC has bad cytocompatibility when the sintering temperature of BGCs is less than 600℃.The Ca/P ratio of the selected porous BGC is 0.47, and the sintering temperature is 680℃. The degradation and biocompatibility properties were verified through degradations and cell compatibility experiments. The 680℃ BGC had been dipped in 37℃ .physiological saline solution for 66h, and pH and the ion concentration of solution were characterized. The result shows there are two phases in dipping process. The degradation rate about ion concentration of 0-6h is about 10 times more than of 6-66h.The 680℃ BGC had been dipped in 37℃, physiological saline solution for 28d, and the weight loss rate and the XRD and SEM figure of dipping BGC were obtained. The weight loss rate is 9.2%, and XRD figure shows that the solubility of MgP2O7and AIPO4 is bigger than β-Ca(PO3) 2.The biocompatibility experiments disclose the porous calcium metaphosphate BGC have good biological compatibility.In all, it is expectative that this kind of porous calcium metaphosphate BGC will be an excellent tissue engineering material.更多还原