【作者】 慕庆峰；

【导师】 赵敏；

【作者基本信息】 东北林业大学 ， 森林保护学， 2005， 硕士

【摘要】 在东北林区的4个自然保护区中共采集、纯化得到25种野生大型白腐真菌,对其氧化酶系进行了定性检测,从中选出6个白腐菌菌种进行染料脱色研究,发现血红密孔菌(Pycnoporus sanguineus)对4大类染料有高效广谱脱色作用。正交试验结果表明在限氮条件下培养5d,其对刚果红脱色率最高可达89.04%;添加微量元素改良培养基的正交试验表明,培养5d最高脱色率可达98.01%;在此基础上添加诱导剂,对刚果红的脱色率则显著降低,经DPS软件处理正交试验数据,发现添加诱导剂愈创木酚是导致脱色率降低的根本原因。使用不同载体固定血红密孔菌降解刚果红,活性炭载体5d脱色率达77%,海藻酸钠载体5d脱色率达56%,游离菌丝5d脱色率达58%。在固定床中分别用海藻酸钠,聚乙烯醇固定血红密孔菌(Pycnoporus sanguineus)进行染料脱色实验,前者对刚果红7d脱色率达92.86%,后者对刚果红7d脱色率达22.97%。实验发现微胶囊粒径的大小与染料脱色有密切关系。设计了一个简易微胶囊发生器,能制备300um的微胶囊。在不添加诱导剂藜芦醇前提下,获得4种染料的6d紫外可见吸收图谱,没有证据表明亚甲基蓝降解,但是血红密孔菌生物量却增加了,而且其他3种染料均有不同程度的降解。更多还原

【Abstract】 Twenty-five kinds of big wild white rotten fungi were collected and purified from four nature-protected areas in the northeast of China. A range of oxidases of these fungi were examined in quality.Six species of white rotten fungi were seilected to use in the study of decolorization.A high efficient and wide range decolorization fungus,Pycnoporus sanguineus was found in the decolorization of four kinds of dyes.Orthogonal test indicated decolorization rate of gongo red could reach as high as 89.04% under the nitrogen-limited condition for five days;The decolorization rate could reach as high as 98.01% when the trace elements were added to the culture medium;0n this basis,the dye (Gongo red)’s decolorizion rate obviously failed during the five days when we added revulsant to the culture medium.Data of orthogonal test was dealt with DPS software,the result showed the ultimate reason was that the guaiacol was added to the culture medium.We used various carriers to immobilize Pycnoporus sanguineus to decolor Gongo red,then the active carbon carriers’ decolorization reached 77%,the sodium alginate carriers’ decolorization reached 56% and the free mycelium’s decolorization reached 58% in five days.The dye decolorization tests were carried out on Pycnoporus sanguineus which was immobilized by sodium alginate and polyvinyl alcohol respectively in the fixed bed column.The result showed that the former had a higher decolorization rate to Congo red,which was 92.86%,while the latter was only 22.97%.Close relationship between microcapsules diameter and decolorization had been declared.A kind of microcapsules creator which could create microcapsules of 300um had been designed.Without veratryl alcohol,UV spectrum of four kinds of dyes had been detected in six days.No certification indicated that the methylene blue was degraded.Yet biomass of Pycnoporus sanguineus had been found increased. Meanwhile other 3 dyestuffs had degraded in different levels.更多还原