补肾活血中药复方对实验性糖尿病大鼠视路神经元病理损害的影响及机理探讨

【作者】 秦伟；

【导师】 谢学军；

【作者基本信息】 成都中医药大学 ， 中医眼科学， 2005， 硕士

【摘要】 目的:观察实验性糖尿病大鼠视路神经元组织形态学的病理改变,并探讨补肾活血中药复方防治实验性糖尿病大鼠视路损害的作用机理。 方法:选用SD大鼠,采用链脲佐菌素(streptozotocin,STZ)建立实验性糖尿病模型。实验大鼠随机分为7组:正常对照组、阴性对照组(模型组)、阳性对照Ⅰ组、阳性对照Ⅱ组、中药低、中、高剂量组。以Nissl染色法和免疫组织化学方法染色显示视网膜、外侧膝状体(exteral geniculate body,EGB)、视皮质(visual cortex,VC)中Nissl小体、神经营养因子—3(neurotrophic-3,NT-3)、突触素Ⅰ(synapsin Ⅰ,Syn Ⅰ)、B淋巴细胞瘤/白血病-2 基因(B cell lymphoma/leukemia-2,Bel-2)的表达,应用Mias-2000图形分析系统对其进行测定和分析。 结果:(1)在病程6个月时,糖尿病大鼠视网膜、EGB和VC中Nissl小体、NT-3、Syn Ⅰ、Bcl-2的阳性染色均较正常对照组有不同程度的降低(P<0.01,0.05);(2)与阴性对照组比较,中药各剂量组大鼠视网膜、EGB和VC中Nissl小体、NT-3、Syn Ⅰ、Bcl-2的阳性染色均有增高,尤以中药高剂量组明显(P<0.01)。 结论:实验性糖尿病大鼠在高血糖状态持续6个月后,其视路神经元发生不同程度的退行性改变;补肾活血中药复方可通过增强糖尿病大鼠视路中NT-3、Syn Ⅰ及bcl-2的表达,来减轻糖尿病大鼠视路神经元的退行性改变,增强其存活能力,并改善神经递质释放调节功能,从而减轻糖尿病性视路损害,这可能是补肾活血中药复方减轻糖尿病视功能损害的重要机制之一。更多还原

【Abstract】 Objective: To observe the histomorphological pathology change of the optic pathway neuron in the experimental diabetic rat, and to probe into the mechanism of Bu Shen Huo Xue Zhongyao Fufang (补肾活血中药复方) to prevent from the lesion of the optic pathway of the experimental diabetic rats.Methods: The diabetic SD rats were made by abdominal streptozotocin-injecting. Experimental rats were divided into normal group, negative control group, positive control groupⅠ , positive control groupⅡ, Bu Shen Huo Xue Zhongyao Fufang groups (lower dosage, moderate dosage, high dosage) at random. And then The Nissl body, NT-3, SynI, Bcl-2 in retina, EGB and VC 17 area were checked by Niss staining method and immunohistochemical staining method. The experimental data were done by SPSS 11.0 software package. Results: (1) The positive expressions of Nissl body, NT-3, Syn I and Bcl-2 in retina, EGB and VC of the rats in the negative control group after 6 months were decreased in various degrees ( P<0.01 , 0.05). (2) in comparison with the expressions of Nissl body, NT-3, Syn I and Bcl-2 in retina, EGB and VC of the rats in the negative control group, those of the rats in the Bu Shen Huo Xue Zhongyao Fufang groups (lower dosage, moderate dosage, high dosage) were enhanced, especially with the high dosages(P<0.01).Conclusions: The experimental diabetic rats which were in high blood glucose condition for 6 months, the optic pathway neuron had the varying degree of the recessive change. By means of strengthening the expression of NT-3, Syn I, bcl-2 in optic pathway neuron to inhibit its recessive change of diabetic rats, Bu Shen Huo Xue Zhongyao Fufang could improve the releasing and regulating function of neurotransmitter to reduce the lesion of diabetic optic pathway.更多还原