【作者】 张贺；

【导师】 苏云明；

【作者基本信息】 黑龙江中医药大学 ， 药理学， 2005， 硕士

【摘要】 胆结石与胆囊炎是危害人类健康的常见病、多发病。两者常相伴发生,且互为病因,纠缠难去,严重影响着人们的身体健康。文献表明,熊胆粉具有清热解毒、抑菌、抗炎、解痉、保肝利胆功效,提示熊胆粉具有治疗胆结石、胆囊炎作用。临床上熊胆粉治疗胆结石、胆囊炎亦取得了良好的疗效。为进一步探讨其作用与机理,为临床治疗提供实验依据,本文进行了相关药效学实验研究。 在综述部分,通过对胆结石、胆囊炎中西医的病因病机和熊胆粉药效学的总结,结合历代医家对胆病的认识,讨论了熊胆粉治疗胆结石、胆囊炎的可行性。 在实验部分,进行了溶石、防石、利胆、解热、抗炎、镇痛等主要药效学实验。实验结果统计分析表明:熊胆粉具有体外溶石作用;能抑制家兔食饵性胆固醇结石形成;对正常及病理状态家兔具有明显利胆作用;具有改善家兔胆汁成分:抑制二甲苯所致小鼠耳廓肿胀:抑制醋酸所致小鼠毛细血管腹腔通透性增高;抑制蛋清所致大鼠足跖肿胀;抑制大鼠肉芽肿;对2,4-二硝基苯酚所致大鼠发热有明显的解热作用;对热板刺激所致小鼠疼痛反应及对醋酸所致小鼠扭体反应有明显的抑制作用。 总之,熊胆粉在溶石、防石、增加胆汁分泌、改善成石胆汁成分、抗炎、镇痛方面均有明显的作用。且均优于阳性对照药利胆排石片。进一步为熊胆粉用于临床治疗胆结石、胆囊炎提供了实验依据。更多还原

【Abstract】 Cholelithiasis and cholecystitis are common and frequently-occurring diseases. Both diseases are always concomitant, which seriously affects the people’s life and health. Reference indicate that bear bile has obvious effect of clearing pathologic heat and toxin,anti-virus,anti-bacteria,anti-inflammation,antispasm,hepatoprotective,choleretic and can be used as an effective Chinese medicine to treat cholelithiasis and cholecystitis. In order to discuss its mechanism and offer basal experimental data for clinic,some experimental study on the pharmacodynamics of bear bile was done in this thesis.In the experimental research part,we observe the datum through the experiment of resolving-stones,excluding-stones, defending-stones,choleretic , antipyretic and anti-inflammation. The results show that (1)bear bile has litholysis efficacy in vitro. (2)It can restrain the formation of bile-stone of rabbit with cholesterol stone induced by food.(3)It has significant choleretic effect on rabbit both normal and pathologic.(4)It can ameliorate the composition of rabbit’s bile.(5)It can inhibit the mice auricle tumefaction degree induced by xylene and the rats footpad swelling induced by egg white.(6)It has significant antipyretic effect on fevered rats induced by 2,4-dinitrophenol.(7)It has significant analgesic effect on mice in writhing test and hot plat test.In conclusion, it has significant effect on resolving-stones, excluding-stones,defending-stones,anti-inflammation and analgesic effect, which is prior to the control group. This conclusion confirms bear bile’s treatment on cholelithiasis and cholecystitis.更多还原