子囊菌DS-9701细胞培养特性及细胞产物的研究

【作者】 万其兵；

【导师】 刘丽丽；

【作者基本信息】 天津师范大学 ， 细胞生物学， 2005， 硕士

【摘要】 本论文对子囊菌DS-9701菌体细胞进行了破壁方法的选择研究,并釆用SDS-PAGE分析方法研究了子囊菌DS-9701在不同培养温度下生长,其细胞可溶性蛋白质表达的差异性。同时以正交设计的方法研究了各种营养条件对子囊菌DS-9701细胞生长的影响。结果获得了较理想的破壁效果,子囊菌DS-9701细胞经破壁处理后,菌丝细胞全部破碎,生殖细胞(分生孢子)残余数分别为 0.132、0.178、0.115和0.239。SDS-PAGE分析表明在不同的生长条件下,子囊菌DS-9701菌体细胞内可溶性蛋白质的表达存在显著差异。各种营养条件也影响子囊菌DS-9701菌体细胞的生长特性。 本文还对子囊菌DS-9701细胞液体深层培养产生红色素的培养基进行了优化研究,获得最佳产生红色素的培养基配方和细胞液体深层培养产生红色素的过程曲线及菌体对数生长曲线,确定了细胞液体深层培养产生红色素的最佳培养条件。更多还原

【Abstract】 We carried out the optional research of the method that be acceptable for breaking up the cell wall of Ascomycetes DS-9701 .and used analysis of SDS-PAGE to research expressional distinction of soluble protein of cell during Ascomycetes DS-9701 was cultivated under different temperatures. Meantime researched cell growing effect of Ascomycetes DS-9701 under a variety of nutrient condition with mothed of direct cross design. We got good result about breaking up the cell wall, the mycelial cell was entirely broken up when the cell of Ascomycetes DS-9701 was disposed by breaking up the cell wall,the leaving numbers of generative cell were 0.132 and 0.178 and 0.115 and 0.239.The analysis of SDS-PAGE show it exists certain distinction that the expression of the soluble protein of cell.there are effects about cell growing character of Ascomycetes DS-9701 under a variety of nutrient condition.we carried out the experiment that optimizes the medium that cultivated cells of Ascomycetes DS-9701 in deep solution for producing the red pigment,got the optimal formulation of medium for producing the red pigment. got curves of culture process and logarithmic growth of thallus about cells of Ascomycetes DS-9701 was cultivated in deep solution for producing red pigment,ascertained optimal cultivating condition about cultivated the strain producing red pigment in deep solution.更多还原