【作者】 闫瑾；

【导师】 崔志清；

【作者基本信息】 天津医科大学 ， 药理学， 2005， 硕士

【摘要】 背景:肠梗阻是腹部外科临床常见的急腹症之一,可由多种病因引起,易反复发作,严重影响患者生活质量,甚至危及生命。中医理论认为“六腑以通为用”,通降下行为顺,滞塞上逆为病;气机不畅,通降失和为病,“不通则痛”,据此采用通里攻下法,应用大承气汤治疗肠梗阻取得了良好疗效,并且开发出了新型中药制剂—大承气颗粒。大承气汤治疗肠梗阻的作用机理的研究已比较深入,但新近研制的大承气颗粒对大鼠不完全性肠梗阻小肠上皮细胞保护作用及机理的研究未见报道。目的:研究大承气颗粒对大鼠不完全性肠梗阻小肠上皮细胞保护作用及机理。方法:(1)模型建立:采用贯穿缝扎末端回肠肠管的方法建立大鼠不完全性小肠梗阻模型。(2)动物分组:随机将大鼠分为正常组、假手术组、模型组、赋形剂组、大承气颗粒剂量组(按生药剂量计算):低剂量组(0.98 g/kg)、中剂量组(2.94 g/kg)、高剂量组(8.82 g/kg)、和阳性对照药乳果糖组(1.34 g/kg)。模型组和给药组行开腹术及用圆针和丝线将回肠肠管1/2横断面结扎,造成不完全性肠梗阻;正常组不作任何处理;假手术组仅行开腹术及用针线穿透肠管而不结扎。各给药组均于手术前30min灌胃给药,造模后连续三天每日定时灌胃给药,正常组、假手术组和模型组给予等体积的生理盐水。(3)指标测定:观察肠梗阻72h的小肠组织形态学、组胺酶活性、肠上皮细胞凋亡情况、小肠粘膜上皮细胞内钙离子水半及血浆与小肠组织NO、MDA、SOD的变化。结果:(1)与假手术组比较,小肠粘膜损伤评分均有增加;大承气颗粒中、高剂量组小肠粘膜损伤评分显著低于模型组;与乳果糖组相比无统计学差异。(2)与模型组比较,大承气颗粒中、高剂量组可降低血浆组胺酶含量(P<0.05或P<0.01)及增强小肠粘膜组胺酶含量(P<0.01);与乳果糖组比无统计学差异(P>0.05)。(3)肠上皮细胞凋亡:与模型更多还原

【Abstract】 BACKGROUND: Intestinal Obstruction can be brought by many causes and it is one of common diseases in surgery practices. The repetitive outbreaks disturb the peace of people’s living and even endanger their lives. There is saying in traditional Chinese Medicine, "If the viscera are not expedite, people will suffer from it ." The judgment of traditional Chinese Medicine accords with the pathogenesis of intestinal obstruction, therefore, method of Tongligongxia is applied. Dachengqi decoction is the traditional Chinese Tongligongxia herbs, and practices also prove that Dachengqi decoction have a good effect in treating incomplete intestinal obstruction. Now new type of preparation -Dachengqi granules has developed . Profound studies has carried out on their mechanisms. However, their protective effect against injury of the Intestinal Epithelial Cells (IEC) induced by incomplete intestinal obstruction has not been testified. OBJECTIVE: Study on the protective effects of Dachengqi granules against injury of IEC induced by incomplete intestinal obstruction in rats. METHODS: (1) Model Establishment: The 1/2 terminal ileum of rat was ligated as incomplete intestinal obstruction model. (2) Groups: Rats were randomly divided into groups: normal group, sham operated group, model group, excipient group, dachengqi granules groups: low dose(0.98 g/kg), middle dose(2.94 g/kg), high dose(8.82 g/kg) and Lactulose group(1.34 g/kg). Half-hour before the operation, drugs were given to the corresponding therapeutic groups, and then were continued at the same time for three days. Saline was given to the rats of normal, sham-operated and model groups according to the same method. For model and therapeutic groups, the intestines were made pinprick and clamped. For sham operated group, the intestines were only made pinprick. Normal group did nothing. (3) Items of Observation: assess and grade histological mucosal damage on the six point更多还原