【作者】 吴丽；

【导师】 魏伟；

【作者基本信息】 安徽医科大学 ， 药理学， 2005， 硕士

【摘要】 目的:探讨SQDG对CCl₄致小鼠急性化学性肝损伤、大鼠肝纤维化及体外原代肝细胞（hepatocytes,HC）损伤模型的保护作用及可能机制,并对SQDG与白芍总苷（total glucoseides of paeony,TGP）、黄芪总皂苷（astragalosides,ASTs）单独用药的特点进行比较。 方法:建立CCl₄致化学性小鼠急性肝损伤、大鼠肝纤维化和体外HC损伤模型,分光光度法检测血清中丙氨酸氨基转移酶（alanine aminotransferase,ALT）、天门冬氨酸氨基转移酶（aspartate aminotransferase,AST）、一氧化氮（nitric oxide,NO）和肝匀浆中羟脯氨酸（hydroxyproline,Hyp）、丙二醛（malondiadehyde,MDA）、超氧化物歧化酶（superoxide dismutase,SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）含量,放免法检测血清中透明质酸（hyaluronic acid,HA）、层粘蛋白（Iaminin,LN）、Ⅲ型前胶原（procollagen Ⅲ,PCⅢ）、Ⅳ型胶原（collagen type Ⅳ,CⅣ）和肿瘤坏死因子-α（tumor necrosis factor-α,INF-α）水平,MTT法检测HC存活率。HE染色法对肝脏组织作病理检查。 结果:1.SQDG对CCl₄诱导小鼠急性肝损伤的保护作用;SQDG能显著降低血清ALT、AST水平;病理组织学结果表明,SQDG(60,120,240 mg·kg^-1)均能使CCl₄诱导的肝细胞损伤减轻。进一步研究发现SQDG(120,240 mg·kg^-1)可降低肝匀浆CCl₄升高的MDA水平,并且升高SOD和GSH-Px酶活性;2.SQDG对CCl₄诱导大鼠肝纤更多还原

【Abstract】 Objective To study the therapeutic effects and mechanisms of SQDG on carbon tetrachloride -induced chemical liver injury in mice , hepatic fibrosis in rats and primary cultured rat hepatocytes as well as its possible mechanisms . At the same time the pharmacodynamics of SQDG was compared with total glucosides of paeony （TGP） or astragalosides （ASTs） of effective dose.Methods The model of carbon tetrachloride -induced chemical liver injury in mice, hepatic fibrosis in rats and primary cultured rat hepatocytes was prepared. The levels of alanine aminotransferase（ALT）, aspartate aminotransferase（AST） , nitric oxide（NO） in serum, hydroxyproline content, malondiadehyde（MDA） content, superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） activities in liver homogenate were assayed by spectrophotometry; serum hyaluronic acid （HA）、 laminin （LN） procollagen III （PCIII）、 collagen type IV （CIV） and TNF-a activity were determined by ridioimmunoassay. The survival rate of CCI4- injury hepatic cell was assayed by MTT dye reduction. Meanwhile, hepatic pathological examination was observed. Results 1 Protective effect of SQDG on carbon tetrachloride-induced chemical liver injury: SQDG (60, 120, 240 mg.kg^-1)was able to significantly decrease serum更多还原