【作者】 郝兴宇；

【导师】 张金桐；

【作者基本信息】 山西农业大学 ， 生物化学与分子生物学， 2005， 硕士

【摘要】 本论文以危害核桃的重要蛀果害虫核桃举肢蛾Atrijuglans hetaohei Yang为研究对象,利用扫描电镜观察极桃举肢蛾雌蛾性信息素腺体和雄蛾触角,确定腺体的部位、表面形态及雄蛾触角感觉器类型。通过室内和野外观察,掌握核桃举肢蛾求偶规律及求偶过程中性信息素释放的时辰节律。利用毛细管气相色谱(GC)分析和人工合成性信息素类似物比照,初步确定核桃举肢蛾性信息素活性组分及化学结构。根据已分析的化学成分,采用适当的方法进行性信息素类似物的定向合成。通过林间诱蛾活性试验,验证性信息素类似物的活性。 试验结果表明:核桃举肢蛾性信息素分泌腺位于腹尖末端8～9节之间的节间膜上,为一个由节间膜特化而成的完整上皮环结构,性信息素感受器有毛形感器和刺形感器;核桃举肢蛾羽化后24小时后性成熟,婚飞和交配活动主要在18:00～20:00;核桃举肢蛾性信息素分泌腺提取物中含有与顺-8-十三碳烯-1-醇(Z-8-13:OH)、顺-8-十三碳烯乙酸酯(Z-8-13:Ac)、顺-9-十六碳烯-1-醇(Z-9-16:OH)、顺-9-十六碳烯乙酸酯(Z-9-16:Ac)类似的碳链长度一致的不饱和醇或不饱和酯;通过Wittig反应合成核桃举肢蛾性信息素类似物,林间试验结果表明这些类似物具有一定的诱蛾活性。更多还原

【Abstract】 We studied the sex pheromon for Atrijuglans hetaohei Yang that is a destructive insect pest of hetao, whose larvae inflict serious damage by tunneling into tree fruits. The location and structures of the pheromone-producing gland of female Atrijuglans hetaohei Yang and the types of the antennae of male moth were studied by scanning electron microscopic. The circadian rhythms of sexual behavior and the sex pheromone production in A. hetaohei were investigated in both laboratory and the field. The active component and chemicals structures of sex pheromones was identified by means of GC and the contrast between the synthesize analog of sex pheromones and sex pheromones produce by the pheromone-producing gland. We synthesized the analog of sex pheromones, to know if it had attraction in field trapping tests.The experimental results indicated that , the pheromone-producing gland is an eversible fold situated ventrally in the modified intersegmental membrane between the eighth and ninth abdominal segments, antennal sensilla contain sensillum trichodeum and sensillum chaeticum; Most of the moths become sexually mature after one day of emergence. Their nuptial flight and mating took place from 18:00 to 20:00; The analogue of (Z)-8-tetradecenyI acetate (Z-8-13:Ac), (Z)-8-tetradecen-l-ol (Z-8-13:OH), (Z)-9-tetradecen-l-ol (Z-9-16:OH), (Z)-9-tetradecenyl acetate (Z-9-16:Ac) in the female sex pheromone gland extracts of A. hetaohei. The sex pheromone analogue of A. hetaohei can be synthesized by Wittiw reaction,it showed attraction in field trapping tests.更多还原