【作者】 郝会芳；

【导师】 董金皋；

【作者基本信息】 河北农业大学 ， 植物学， 2005， 硕士

【摘要】 本试验以金丝小枣为材料,研究了枣果不同组织中多酚类物质的提取方法。在此基础上,系统分析了金丝小枣、赞皇大枣、阜平大枣枣果生长发育过程中不同组织多酚类物质含量的变化规律,比较了不同品种枣的枣皮、枣肉和枣核中多酚物质的含量差异。同时对不同采摘期金丝小枣枣皮、枣肉、枣核中的多酚类物质粗提物进行了体外抗氧化能力的测定。主要结果如下: 1、枣皮及枣肉多酚物质最佳提取工艺是:40%乙醇,料液比1:30,每次浸提60min,60℃下浸提两次。枣核多酚物质最佳提取工艺为:料液比1:30,pH3.0的80%乙醇,50℃下每次浸提12h,浸提两次。 2、枣果生长发育过程中,金丝小枣枣皮多酚含量在采摘初期含量最高,以后平缓下降,白熟期又出现一高峰,果实成熟时降至最低。赞皇大枣枣皮随着枣果的发育多酚含量逐渐增高,白熟前期含量最高,以后逐渐下降,果实成熟时含量最低。阜平大枣枣皮多酚含量在整个生长期变化不大。 在枣核和枣肉中,尽管金丝小枣、赞皇大枣、阜平大枣的多酚含量存在差异,但均为采摘初期多酚含量最高,随果实成熟多酚含量逐渐降低。 3、在整个生长周期中,金丝小枣枣皮中的平均多酚含量远远高于枣肉,枣核中的多酚含量居中;赞皇大枣中枣核的平均多酚含量最高,为88.88mg/mL,而枣肉中的平均多酚含量仅为1.53mg/mL,枣皮平均多酚含量居中,为42.34mg/mL:阜平大枣枣核及枣肉的平均多酚含量与赞皇大枣相当,枣皮的平均多酚含量居中,为20.63mg/mL。 4、不同枣品种相同部位的多酚含量存在很大差异。枣皮中,赞皇大枣中的多酚含量最高,依次是金丝小枣、阜平大枣。枣肉中,金丝小枣多酚含量最高,依次是阜平大枣、赞皇大枣。做为枣产品加工过程中的废弃物—枣核,其中的多酚物质含量为金丝小枣含量最低,赞皇大枣枣核中的多酚含量最高,而阜平大枣的多酚含量居中。 5、利用BPCL_L发光分析仪和紫外可见分光光度计,测定了用最佳提取方法得到的金丝小枣各组织多酚粗提物的体外抗氧化活性。结果表明,不同采摘期所得不同部位多酚粗提物对羟自由基、亚硝酸根、DPPH·及亚油酸的脂质过氧化均具有抑制作用,同时对由·OH引起的DNA损伤也具有较好的保护作用。更多还原

【Abstract】 The extracting technology of polyphenolic compounds from the different parts of "Jinsixiaozao" has been studied. Based on this, we did some research on the content change of polyphenols compounds in different parts of 3 cultivars of Chinese jujube(Ziziphus jujuba Mill.),"Jinsixiaozao","Zanhuangdazao" and "Fupingdazao" during the fruit development, and compared the content of polyphenolic compounds in skin, flesh and core from the three cultivars. Then the antioxidant activity in vitro of the crude polyphenolic extracts from the three parts of the fruit at different periods were assayed. The main results are as follows:1. The optimum condition for extracting polyphenolic compounds of the skin and the flesh from Chinese jujube were determined to be 40 % ethanol at 60 ℃ water bath for 60 min and solid to liquid ratio 1:30 for twice. And the optimum condition of the core from Chinese jujube were determined to be 80% of pH3.0 ethanol at 50 ℃ water bath for 12 h and solid to liquid ratio 1:30 for twice.2. During the development of the jujube fruit, the content of polyphenolic compounds in the skin is high in the initial picked stages and the white-ripe periods of "Jinsixiaozao", but "Zanhuangdazao" is high in the prophase of white-ripe periods. The content of polyphenolic compounds has no evident change from the skin of "Fupingdazao".In the flesh and the core, though there are some differences among the content of polyphenolic compounds from the three cultivars, it is high in the initial picked stages, up to fruit ripe the content decreased slowly.3. In the cycle of growing entirely, the average content of polyphenolic compounds in the skin of "Jinsixiaozao" is much higher than the flesh, and the core is between two parts. In "Zanhuangdazao" and "Fupingdazao", the content of polyphenolic compounds from the core of the fruits are the highest, then are the skin and the flesh, respectively.4. A great difference existed in the content of polyphenolic compounds in the same part of different Chinese jujube cultivar. The content of polyphenolic更多还原