【作者】 简洁；

【导师】 梁钢；

【作者基本信息】 广西医科大学 ， 药理学， 2005， 硕士

【摘要】 目的:筛选复方茶多酚的最佳组方并进行一般药理学研究。方法:采用正交设计法,将广西绿茶、银杏、三七、丹参等四种活性提取成分有机组方制成复方茶多酚(TPC),采用最大耐受量试验考察其急性毒性;采用耐缺氧试验考察其耐缺氧效力;采用HPLC 法进行组方初步稳定性研究。结果:初步筛选出TPC 最佳组方;7g.kg-1.d-1剂量给小鼠连续i.g.7d 无毒性反应发生;175、350、700mg.kg-1.d-1 剂量的TPC 连续i.g.7d,均明显增强小鼠的耐缺氧能力(P<0.01)并呈剂量依赖性,其中高剂量组效果最好,与空白对照组比较,小鼠耐缺氧时间延长48.93%;以EGCG 含量为指标,在2h 内等间隔时间对高剂量配比TPC 溶液进行五次含量测定,RSD 值为1.98% 。结论:本课题组初步筛选出TPC 最佳组方;TPC 低毒;TPC 能明显增强小鼠的耐缺氧能力;TPC 溶液室温下放置2h 稳定。更多还原

【Abstract】 Objective:To screen TPC and study it’s general pharmacolog effects. Methods:Orthogonal design was used to prepare TPC with TP ,Egb761 , PNS ,DS. Themaximal-tolerant-dosage method was used to observe the acute-toxicity of TPC.Anoxic tolerance was measured by antihypoxia method .The initial stability ofTPC was measured by HPLC . Results: The best compound of TPC was screened.TPC was low toxicity and all mice were alive with i.g. 7 g.kg-1.d-1 of TPC for 7d .The anoxic tolerance of mice increased significantly with dosage dependence byi.g. 175、350、700 mg.kg-1.d-1 of TPC respectively. Among the total,the high dosagewas best.Comparing with the vacuo , the survival time of mice elongated 48.93%.TheEGCG’s content of TPC was measured at regular intervals for five times within 2hby HPLC , and the RSD was 1.98% .Conclusions: The best compound of TPC isscreened in preliminary experiment.TPC is low toxicity. TPC can increase theanoxic tolerance of mice. The TPC solution is stable within 2h at roomtemperature.更多还原