高强度聚焦超声对兔腹腔神经节阻滞的实验研究

【作者】 曾令全；

【导师】 魏安宁；

【作者基本信息】 重庆医科大学 ， 麻醉学， 2005， 硕士

【摘要】 目的:本实验分别运用乙醇、HIFU 以及利多卡因联合 HIFU 阻滞兔 CG,然后观察并比较在三种方式下血压改变以及乙醇、HIFU 阻滞神经节后的形态结构改变,为临床安全运用 HIFU 作为神经阻滞剂治疗上腹部癌痛提供实验依据。 方法:将 21 只新西兰大白兔随机分为三组(A,B,C),每组各 7只,麻醉后开腹,然后解剖并暴露 CG,A 组用 95%的乙醇阻滞,B 组运用 HIFU 照射 CG,C 组用利多卡因+HIFU 阻滞,采用 BL-410 生理机能实验系统连续记录兔的血压,比较各组在阻滞后 0,1,3,5,10min 的血压变化。实验完毕随机从 A、B 两组各选取 2 只取 CG 作光镜观察。从 A 组随机抽取一只行灌注固定取神经节作电镜观察。 结果:(1)形态结构的改变:A 组大部分神经元核仁模或消失,核缩小,染色加深。B 组神经元细胞出现核仁消失或模糊,核缩小,染色加深,胞核偏位,同时有较大空泡形成。(2)血压变化:A 组在神经节被浸润乙醇后 3min 出现血压明显的下降(P<0.01),B 组在神经节被 HIFU 阻滞的即刻,血压显著下降。C 组血压无明显变化。B 组与C 组相比,在各相应时间点 B 组血压下降显著(P<0.01)。A 组与 B 组间在相应时间点 0 min,1min 时有非常显著性差异(P<0.01),在 3min时有显著性差异(P<0.05)。 结论:应用 HIFU 较 95%的乙醇阻滞 CG 对血流动力有更大的影响。其原因可能与 HIFU 对神经节有更明显的损伤有关。更多还原

【Abstract】 Objective:Alcohol and HIFU and Lidocaine combined with HIFU were respectively used for blocking the CG of rabbit in this experiment, then we observed and compared the changes of Bp between three means as well as the changes of ganglionic morphology induced by alcohol and HIFU, so provide the experimental basis for safely using HIFU to block CG to treat the pain of epgastric region terminal cancer. Mothods: 21 New Zealand rabbits were randomly divided into three groups(A, B, C ). Dissected and exposured CG , then the CG of group A was blocked by alcohol, B was expoursed to HIFU (25W/cm2 10s), C was blocked by Lidocaine and HIFU(25W/cm2 10s), Using BL-410 Biological Function System continuously record the changes. Compare the changes of Bp at 0, 1, 3, 5, 10min after CG being blocked . Resected CG for morphologic observation finally. Results: The changes of ganglionic morphology of B were severe compared B. The Bp of A was descend at 3min(P<0.01), B was descend at 0min(P<0.01), C has no obvious changes all along. The Bp of B was descend more remarkable at every time point compared C. A was descend more remarkable at 0min, 1min(P<0.01). Conclusion: HIFU blocking CG have more influence on homodynamic than alcohol. It maybe correlate with the more obviouse injue to CG that prouduced by HIFU.更多还原