【作者】 顾军；

【导师】 王雅杰；

【作者基本信息】 第二军医大学 ， 肿瘤学， 2005， 硕士

【摘要】 目的:检测WWOXmRNA和编码的蛋白在在肝外胆管癌中的表达并分析其临床病理学意义。 方法:用RealTime RT-PCR方法检测21例胆管癌组织及5例正常胆管组织WWOXmRNA表达情况,用免疫组化方法检测相应组织的蛋白表达情况。 结果:WWOX mRNA在肝外胆管癌中的表达显著低于正常胆管组织(t=10.145,P<0.001),表达缺失为47.16%;免疫组化染色阳性为9例,阴性为12例,蛋白缺失率为57.14%。其mRNA及蛋白表达与年龄,性别,手术前的肝功能情况及临床分期相关无统计学意义,而与组织学分级相关有统计学意义(r分别为-0.583和-0.840,p值分别为0.007和<0.001)。WWOXmRNA和蛋白在分化差的胆管癌组织中的表达显著低于分化较好的肿瘤组织。 结论:WWOX表达改变参与胆管癌的发病,表达减少和组织学分级相关,可作为预测肝外胆管癌生物侵袭性的有效指标,将来可发展成为诊断和治疗的一个靶点。更多还原

【Abstract】 Object: To evaluate the clinico- pathological significance of WW domain-containing oxidoreductase messenger RNA and the protein expression in human extrahepatic cholangiocacinoma(ECC).Method: The clinicopatholigical significance of WWOXmRNA expression in 21 patients with extrahepatic cholangiocarcinoma and 5 cases of nomal bile duct tissue was examined. The WWOXmRNA expression in extrahepatic cholangiocarcinoma and normal bile duct tissue was determined using RealTime reverse transcription polymerase chain reaction analysis. The WWOX protein expression in extrahepatic cholangiocarcinoma and normal bile duct tissue was determined using immunohistochemical staining.Result: Expression of WWOX messenger RNA and the protein was lower in tumor tissue than in the normal bile duct tissue ,and loss of messenger RNA was observed in 47% of cases (t=10.145, P<0.001).Loss of WWOX protein expression was found in 57% of ECC adenocarinoma primary specimens. Both of the messenger RNA expression and the protein expression has a significance of correlation with histological grading, coefficient correlation is -0.583 and -0.840 respectively,while has no correlation with other clinicopathological factors such as age,sex,clinical stage or the status of hepatic function in preoperation. The expression of messenger RNA and the protein was significant lower in poorer differentiated ECC than moderate differentiated or well differentiated cases.Conclusion: Alterations of the WWOX gent may be involved quite frequently in cholangiocarcinoma tumorigenesis. Reduced expression of WWOX is associated with the histological grade of ECC and may therefore prove to be a useful marker for predicting the biological aggressiveness of human ECC. The data could be used in future studies to develop diagnostic and targeted therapy of bile duct cancer.更多还原