【作者】 郑毅；

【导师】 徐凯；

【作者基本信息】 安徽农业大学 ， 果树学， 2005， 硕士

【摘要】 以抗性不同的五个草莓品种:丰香(不耐寒)、明磊(耐寒)、爱美(不耐热)、硕露(耐热)和鬼怒甘(耐寒耐热)盆栽苗为试验材料,在 25℃的条件下预培养10d 后,以 25±1℃下培养 25d 为对照,丰香、明磊、鬼怒甘分别在 10±2℃和 0±2℃的环境下培养 7d;爱美、硕露、鬼怒甘分别在 30±2℃和 38±2℃的环境下培养7d,然后再在 25±1℃下培养 7d 作为低温、高温胁迫的恢复处理。研究了温度胁迫对草莓叶片光合机构运转的影响,及植株本身的自我保护机理。 与对照(CK)相比,低温、高温胁迫均使草莓叶片光合速率(Pn)下降,而且测得的表观量子效率(AQY)、羧化效率(CE)、最大的光化学效率(Fv/Fm)、光化学猝灭系数(qP)及光系统 II 的量子产量(ΦPSII)均下降,相反,光呼吸速率(Pr)和非光化学猝灭系数(qN)上升。 品种间比较发现,低温胁迫下,耐寒品种比不耐寒品种受到的伤害要轻;高温胁迫下,耐热品种比不耐热品种受到的伤害要轻。光呼吸及依赖于叶黄素循环的热耗散是草莓在温度胁迫下的主要自我保护机制。 以 Western-blotting 蛋白质印迹分析了在 5℃和 38℃下强光对丰香草莓叶片 D1蛋白周转的影响。结果表明,强光处理一周(每天处理 4 小时)后,高温强光下D1 蛋白降解加快,低温抑制了 D1 蛋白合成与降解,而高温与强光的共同胁迫加剧了 D1 蛋白的降解。更多还原

【Abstract】 Potted plants of five strawberry varieties differing in temperature resistivity(FengXiang(not cold-resistant), MingLei(cold-resistant), AiMei(not heat-resistant),ShuoLu(heat-resistant) and GuiNuGan(cold&heat resistant))were subjected to 25±1℃(control) , FengXiang, MingLei, GuiNuGan: 10±2℃、0±2℃(low temperature stress),AiMei,ShuoLu,GuiNuGan: 30±2℃、38±2℃(high temperature stress), 800~1000μmol·m-2·s-1 PFD and 10 hours photoperiod during 7 days and recovered during 7 days at25±1℃ at the same light regime. The effects of temperature stress on the operation ofphotosynthetic apparatus and the protective mechanism in leaves of strawberry plantwere studied. Compared with control, net photosynthetic rate (Pn), apparent photosyntheticquantum yield (AQY), carboxylation efficiency (CE), photochemical efficiency (Fv/Fm),photochemical quenching (qP) and quantum yield of PSII photochemistry (ΦPSII) alldeclined under temperature stress. On the other hand, photorespiratory rate(Pr)and no-photochemical quenching (qN ) increased under temperature stress. Compared the five strawberry varieties, we found that under low temperature stress,the injury to cold-resistant variety was lower than that in not cold-resistant variety, andthe injury to heat-resistant variety was lower than that in not heat-resistant variety underhigh temperature stress.Photorespiration and xanthophyll cycle were the major protectivemechanism under temperature stress in strawberry leaves. The effects of high light stress(1800 μmol·m-2·s-1) under 5℃ and 38℃ to D1protein turnover were studied by Western-blotting in strawberry leaves. The resultindicates, after a week treatment with high light(4 hours a day),the degradation of D1protein under 38℃ was more than 5℃. Low temperature stress induced an inhibition ofD1 protein synthesis and . degradation. the degradation of D1 protein was acceleratedunder combined stress of heat and high light.更多还原