栝楼桂枝汤化裁对戊四氮点燃癫痫大鼠大脑内ATPase及丙二醛（MDA）水平影响的实验研究

【作者】 经浩宇；

【导师】 艾华；

【作者基本信息】 辽宁中医学院 ， 中医临床基础， 2005， 硕士

【摘要】 目的:用实验研究的方法,从神经递质角度探讨癫痫的发病机制及栝楼桂枝汤治疗癫痫的作用机理。方法:选用成年Wistar大鼠,按性别、体重分层后,再随机分为四组(正常组、模型对照组、栝楼桂枝汤组和卡马西平组)。采用Dihel癫痫模型制作方法并加以改进,进行癫痫模型点燃。按Smialowki的癫痫分级标准进行分级,测定大脑内Na~+-K~+-ATPase、MDA水平。结果:1) 栝楼桂枝汤组和卡马西平组大鼠癫痫的发作级别低于模型对照组(P<0.05);2) 栝楼桂枝汤组和卡马西平组癫痫大鼠大脑内的Na~+-K~+-ATPase含量高于模型对照组,MDA水平低于模型对照组(P<0.05);栝楼桂枝汤组和卡马西平组相比较,无显著差别(P>0.05)。结论:栝楼桂枝汤能够降低癫痫的发作级别;降低大鼠脑组织中MDA的含量,减轻自由基对细胞膜的攻击,提高膜Na~+-K~+-ATPase活性,稳定膜电位,具有一定抗癫痫作用。更多还原

【Abstract】 object: To probe the pathogenesis of epilepsy in the aspect of neurotransmitter, demonstrate the clinical effect of DTC by experimental method and study its mechanism of action in epilepsy. Methods: 40 adult Wistar rats were divided into four groups at random (stratified according to their sex and weight) : normal group, model groups , Chinese herbs group and Western medicine group. A week later, all the rats were include to epilepsy by PTZ by the improved Dihel epileptic model method . The grade of onset was determined by Smialowki standard after kindle and their level of Na+-K~+-ATPase and MDA of all the epileptic brains were detected. Result : the level of Na+-K+-ATPase of all the epileptic brains were decreased and the level of MDA increased compared with those of normal group ( P <0.05),DTC can lower the onset grade of epilepsy (P <0.05) and increase Na*-K*-ATPase and decrease MDA in epileptic rats brains . Conclusion : The activity of ATPase and liquid peroxide may be primary causes for epilepsy. DTC can reduce the attack grade, improve the activity of Na+-K+-ATPase and prevent liquid peroxide. The DTC has no significance compared with the carbamazepine group and is an optimal anti-epilepsy prescription .更多还原