【作者】 王增健；

【导师】 焦奎；

【作者基本信息】 青岛科技大学 ， 物理化学， 2005， 硕士

【摘要】 本论文选取含有苯胺结构的若干有机小分子（碱性品红、邻氨基酚、邻联茴香胺）作为对象,分别对碱性品红的光谱和电化学性质及与DNA的相互作用,聚邻氨基酚/Ni²⁺膜的制备、电催化性质及DNA的固定,邻联茴香胺在铂网栅光透电极上的薄层光谱电化学性质进行了研究。 运用循环伏安法和可见吸收光谱法研究了碱性品红和DNA的相互作用,实验证明碱性品红对dsDNA比对ssDNA具有更高的选择性结合能力,可望应用于选择性区分dsDNA和ssDNA以及在DNA生物传感器中作为杂交指示剂。并制备了聚碱性品红修饰的玻碳电极,DNA在此电极上有电化学响应。 根据聚苯胺薄膜良好的导电性能选择了邻氨基酚作为聚合对象,运用电化学聚合法在Pt电极上制备了嵌入金属离子Ni²⁺的聚邻氨基酚导电聚合物膜（Ni/P-OAP/Pt）,该膜有良好的伏安特性,并且具有三维立体结构。成功地将DNA固定在电极表面。同时利用聚合物膜中Ni²⁺能催化葡萄糖的电氧化的性质,实现Ni/P-OAP/Pt电极对葡萄糖的电催化测定。葡萄糖在此电极上的电催化氧化峰电流与葡萄糖含量在0.03～0.08 m mol·L^-1范围内呈良好的线性关系。 采用电化学和光谱电化学方法,在不同pH值的B-R缓冲溶液中对伏安酶联免疫分析体系中灵敏底物邻联茴香胺（ODA）的电氧化性质进行了研究。发现在oH 2.0 B-R缓冲溶液中ODA的电氧化为2e的一步转移过程,在pH 4.7 B-R缓冲溶液的情况下则为分步的两个单电子的电化学氧化过程。pH 9.0的情况下还原峰基本消失,表现为不可逆的电氧化过程。更多还原

【Abstract】 In this thesis, some derivatives of aniline such as fuchsin basic （FB）, o-aminophenol （OAP）; o-dianisidine（ODA） were selected as the studying objects. The study was involved in spectroscopy, electrochemistry and the interaction of FB with DNA; the fabrication, the electrocatalysis of poly（o-aminophenol）/nickel modified platinum electrode（Ni/P-OAP/Pt）) and the immobilization of DNA on Ni/P-OAP/Pt; and the thin-layer spectroeletrochemistry of ODA at a platinum minigrid optically transparent electrode.The interactions of DNA with fuchsin basic （FB） were studied by spectroscopic and electrochemical methods. The more powerful interaction of FB with dsDNA than with ssDNA was showed in the experiments, which may be expected to be applied in recognition of dsDNA with ssDNA and in DNA biosensor as hybridization indicator. （Poly） fuchsin basic modified electrode was prepared and the electrochemical behavior of DNA on such an electrode was also studied.Poly（o-aminophenol）/nickel modified platinum electrode（Ni/P-OAP/Pt） was prepared by cyclic voltammetric electropolymerization in HClO₄ solution. DsDNA was immobilized onto such electrode via three-dimensional structure of polymer film and electrostatic effect between dsDNA and Ni²⁺ ion. At the same time, the Ni/P-OAP/Pt electrode exhibited an electro catalytic effect on the glucose oxidation. The electrocatalytic oxidation peak current of glucose has a good linear dependence on concentration of glucose.更多还原