【作者】 田润华；

【导师】 马爱国； 梁惠；

【作者基本信息】 青岛大学 ， 营养与食品卫生学， 2005， 硕士

【摘要】 目的 抗氧化营养素维生素E（WE）、维生素c（VC）和β-胡萝卜素（β-c）是人体所需的重要的维生素,在抗衰老、增强机体免疫力、减少DNA损伤等方面的功能已有研究报道,但人体补充多种抗氧化维生素后,对老年人群的脂质代谢水平及肝脏功能的影响尚未见有明确的报导,相关的报导也大多为单一维生素的补充或仅限于动物实验。并且结论时有不符的现象。因此,本研究拟通过VE、VC和β-C营养素的补充,观察老年人群脂质代谢水平的变化,探讨补充β-胡萝卜素对于老年人群脂质代谢水平及肝脏功能的影响。 方法 本研究采用整群抽样的方法选取某农村老年人群共300人,年龄在60-75岁,无严重疾病自愿参加并签定知情同意书者为研究对象,随机分为5组,分别给予A、B、C和D组补充VE200mg/d和VC300mg/d;其中β-C的补充量为A组16.7mg/d,B组8.35mg/d,C组0mg/d,D组5.6mg/d;E组每天仅给予VE5mg;补充时间为16周。补充前后分别采集研究对象空腹静脉血进行相关指标的检测。分析指标包括脂类、酶类两部分。血浆脂类如甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、载脂蛋白AⅠ（Apo-AⅠ）、载脂蛋白B₁00（Apo-B100）及脂蛋白[a]（Lp（a））;酶类指标如谷丙转氨酶（ALT）、谷草转氨酶（AST）、乳酸脱氢酶（LDH）、碱性磷酸酶（ALP）及γ-谷氨酰转肽酶（γ-GGT）;采用相应的临床诊断试剂盒进行测定。通过上述指标的测定分析,观察补充抗氧化营养素前后对老年人群脂质水平的影响以及不同剂量及剂型的抗氧化营养素对老年人群脂质代谢水平的影响。此外,为了解青年和老年人群脂质代谢和肝功能的年龄差别,选择某高校在校大学生,年龄18-25岁共183人,比较青年人群与老年人群脂质各指标的水平和差异。 结果 通过各指标前后改变的对比研究,结果显示抗氧化营养素的补充能显著改善老年人群整体脂质代谢水平,老年人群补充营养素后血浆TC水平、血浆LDL-C更多还原

【Abstract】 Objective Vitamine E, Vitamine C and β -carotin are essential vitamines to human body as antioxide nutrients. They can prevent aging, enhance immunity and reduce DNA damage,etc, which have been broadly confirmed. But it is still unknown that if these vitamines can influence the levels of lipids metabolism and liver function when aged people supplement these antioxide vitamines. Most of the related reports are about supplementation of single vitamine or about experiments in which animals are selected as the subjects. Also these related reports are not always consistent with each other. Thus this study will discuss the influence of these vitamines to the aged people’s lipids metabolism by comparing the results of supplementing vitamine E, C and β -carotin to aged people or not.Methods The study selects 2 groups of people as the subjects. One group are composed of old people aged 60 to 75 years old from a country of Qingdao city. The other consist of college students aged 21 to 24. Fasting venous blood are collected from all the subjects voluntary before the experiments. Aged people are divided into 5 groups in the experiment at random and 4 groups for the younger people. Each group have been supplied compound nrtrients （vitamine E,Vitamine C and β-carotin） each day with different doses 16 weeks for the old and 8 weeks for the young. Then fasting venous blood are collected again to examine related factors,which conclude factors of lipids and liver function, etc. All these factors are detected by clinical diagnostic reagent box respectively.Results By contrasting the results of supplementing vitamines or not , it shows that supplementing different does of antioxide nutrients can improve the total levels of lipids metabolism for aged people. By using t-test the data show that after supplementing the nutrients the levels of TC, LDL-C and Apo-B₁₀₀ decrease obviously by 11%, 38% and 33% respectively, while the level of Apo-A I increases obviously by 15% and the level change little for TG and LP（a）. The levels of the enzymatic factors also change at different extent. The levels of AST, ALT, ALP and γ -GGT increase obviously by 18%, 96%, 82% and 52% respectively.Through further study by dividing subjects into groups it shows that different antioxide nutrients can also influence different factors independently. By ANOVA the level of Apo-A I increases in the group which subjects are given VE 5mg/d contrasted by the 2 groups given VE 200mg/d and VC 300mg/d in which one group is given β -C 5.6mg/d and the other is given no β -C. The level of LDL in the groups given β -C 16.7mg/d and 8.35 mg/d increases contrasted by the no β -C group. Conclusion: Supplementing VE, VC and β -C can improve the levels of lipids metabolism for aged people, which can decrease the levels of TC, LDL and Apo-B₁₀₀ and increase Apo-A I and HDL, which are all beneficial to reduce the incidence of更多还原