【作者】 李昆；

【导师】 吴树清； 呼和巴特尔；

【作者基本信息】 内蒙古农业大学 ， 临床兽医学， 2005， 硕士

【摘要】 本研究采用放射免疫法和荧光分光光度法对秋季、春季两个季节多赛特肉羊和蒙古羊两个品种,每个品种分羔羊、育成羊、成年羊三组,每组各 30 只,共 180只羊的血清、被毛以及抗凝血样品进行了促甲状腺激素、甲状腺激素、微量元素硒、谷胱甘肽过氧化物酶的测定。 结果显示,秋季蒙古羊血清促甲状腺激素和甲状腺激素显著高于多赛特肉羊(P<0.05);蒙古羊血清和被毛 Se 含量显著高于多赛特肉羊(P<0.05);蒙古羊血清GSH-Px 活性显著高于多赛特肉羊(P<0.05);春季多赛特羊血清促甲状腺激素略高于蒙古羊,差异不显著(P>0.05);蒙古羊血清甲状腺激素含量显著高于多赛特羊(P<0.05);蒙古羊血清和被毛 Se 含量显著高于多赛特肉羊(P<0.05);蒙古羊血清 GSH-Px 活性显著高于多赛特肉羊(P<0.05);秋季与春季相比,春季两品种羊血清促甲状腺激素、甲状腺激素、GSH-Px 活性以及被毛 Se 含量均显著高于秋季含量(P<0.05);秋季两品种羊血清 Se 含量高于春季含量,差异显著(P<0.05)。 本实验研究证明了在同等饲养管理条件下,蒙古羊被毛硒含量、血清硒含量以及甲状腺激素、促甲状腺激素和谷胱甘肽过氧化物酶都显著高于多赛特肉羊,而这些指标的差异可能是多赛特肉羊抗病性低于蒙古羊的原因之一。这为根据不同季节调整饲料营养结构,改进饲养管理提供了重要依据更多还原

【Abstract】 Two breeds of sheep, Poll Dorsetl and Mongolian Sheep were used in this study in autumn and spring. Each kind of sheep was divided into three groups that were lamb group, young group and adult group, 30 sheep per group. Thyroid hormone, thyroid-stimulating hormone, Selenium and Glutathione peroxidase were determined by using Radioimmunoassay (RIA) and visible spectrophotofluorometry respectively from the serum samples and wool samples. The results showed that: In autumn,The levels of thyroid hormone and thyroid-stimulating hormone in serum samples of Mongolian Sheep were significantly higher than that of Poll Dorset sheep(P<0.05). The levels of selenium in serum and wool samples of Mongolian Sheep were significantly higher than that of Poll Dorset sheep(P<0.05). The activities of Glutathione peroxidase (GSH-Px) in serum samples of Mongolian Sheep were significantly higher than that of Poll Dorset sheep(P<0.05). In spring, The level of thyroid-stimulating hormone in serum samples of Poll Dorset Sheep was little higher than that of Mongolian sheep(P>0.05). The level of thyroid hormone in serum samples of Mongolian Sheep were significantly higher than that of Poll Dorset sheep(P<0.05). The levels of selenium in serum and wool samples of Mongolian Sheep were significantly higher than that of Poll Dorset sheep(P<0.05). The activities of Glutathione peroxidase (GSH-Px) in serum samples of Mongolian Sheep were significantly higher than that of Poll Dorset sheep(P<0.05). The levels of thyroid hormone , thyroid-stimulating hormone and GSH-Px in serum samples and selenium in wool samples of Mongolian and Poll Dorset Sheep were significantly higher in spring than in autumn(P<0.05). The levels of selenium in serum samples of Mongolian and Poll Dorset Sheep were significantly higher in autumn than in spring(P<0.05). The experiment proved that all the levels of selenium in wool and serum,thyroid hormone, thyroid-stimulating hormone and GSH-Px in serum of Mongolian Sheep were significantly higher than that of Poll Dorset sheep(P<0.05) under the same conditions and this is maybe one of the reasons that the disease resisitivity of Poll Dorset is lower than that of Mongolian Sheep. It is important to perfect element of the nutrition and management of the feeding.更多还原