【作者】 何国英；

【导师】 夏中生；

【作者基本信息】 广西大学 ， 动物营养与饲料科学， 2005， 硕士

【摘要】 本研究主要通过化学分析、小白鼠慢性毒性试验、猪鸡消化代谢试验等方法来评定构树叶(LBP)营养价值。 试验一选取平均体重为29.51±1.08kg杜×长×大三元杂交去势公猪6头,采用肛门收粪法测定构树叶营养物质(能量、粗蛋白、粗脂肪、粗纤维、无氮浸出物)的表观消化率,氮沉积率和蛋白质生物学价值。结果表明,猪对构树叶的能量、粗蛋白、粗脂肪、粗纤维、无氮浸出物的表观消化率分别为68.10%、76.50%、30.13%、32.61%、83.22%,消化能(DE)为10.54MJ/kg,氮沉积率和蛋白质生物学价值分别为61.72%、70.31%。 试验二选取12只健康、体况正常、平均体重为1.53kg的黄羽肉鸡,随机分为4组,每组3只鸡,每只鸡置于特制的铁质代谢笼内,采用TME法测定三黄鸡对构树叶营养物质的利用率。试验结果表明:总能、粗蛋白、粗脂肪、粗纤维和无氮浸出物的真利用率分别为50.71%、89.92%、97.06%、20.55%和68.87%,表观代谢能和真代谢能分别为6.92MJ/kg和8.11MJ/kg。试验证明黄羽肉鸡能较好的利用构树叶中的粗蛋白,对能量的利用率偏低。 试验三选取平均体重18-20g左右,公母各半的昆明系小白鼠64只,随机分为4组,每组16只,进行35天的亚慢性毒性饲养试验。更多还原

【Abstract】 Nutritional values of leaves of Broussonetia papyrifera (LBP) had been evaluated on following experiments。In experiment 1, 6 averaging 29.50kg in body weight, healthy crossbred growing male pigs (DLY) were used to study the apparent digestibilities of nutritients (including GE, CP, EE, CF and NFE),N retention and apparent biological value of protein in LBP through the method of collecting feces from anus. The results showed that the apparent digestibilities of GE, CP, EE, CF and NFE were as follows respectively: 68.10%, 76.50%, 30.13%, 32.61%、 83.22%; DE , N retention and apparent biological value protein in LBP were 10.54MJ/kg , 61.72%, 70.31% respectively.In experiment 2, 12 about 1.53kg yellow broilers were used to study the availabilities of GE, CF, EE, CF, and NFE of LBP through chick TME method. The result showed that the true availability of GE, CF, EE, CF, NFE in LBP were 50.71%, 89.92%, 77.06%, 20.55%, 68.87% respectively. Apparent metabolizable energy and true metabolizable energy were 6.92MJ/kg or 8.11MJ/kg respectively. The experiment suggested that yellow broilers can utilize protein effectively, but not much effectively to energy.In experiment 3, 56 Kunming-mice were randomly divided into 4 groups (16 mice per group) by sex and weight, i.e. group A,B,C,D respectively. The 4 groups received diets containing 0% LBP (control group), 10% LBP, 20%LBP, and 40% LBP respectively for 35 days to evaluate the chronic toxicity of LBP. The resultshowed: ALP of group C and BUN、 ALP of group D were different significantly from group A(P<0.05). ALT of group D were extremely significant differences from group A(P<0.01). The section of liver and kidney of tissues showed: liver and kidney of tissues of group D have slight pathological symptom. Group B and group C have not significant differences.As described above, it was concluded that LBP can be widely used as ingredient of diet of livestock and poultries, but we should control the dose of addition under 20% and improve the energy of diet and keep the balance of nutrients of diet.更多还原