【作者】 黄海英；

【导师】 徐秋萍；

【作者基本信息】 浙江大学 ， 急诊医学， 2005， 硕士

【摘要】 研究背景:急性中毒是急诊医学的重要内容,有机磷农药中毒是较为常见的一种急性中毒。有机磷化合物(OPs)目前在农业上广泛使用,这些化学物质被大量排入环境,对环境产生直接的影响,而且OPs还可通过食物链副集作用进入生物体,对生物体产生更为严重的后果。众所周知,有机磷对生物体的主要效应是抑制胆碱酯酶(AChE)活性,使神经冲动的传导发生紊乱,从而出现一系列急性中毒症状。对于有机磷农药引起的重度中毒症状、中间综合征及迟发性神经病变已普遍得到人们的注意,然而有机磷农药还可诱导机体氧化应激,细胞凋亡及坏死等等,这些生物学效应往往是潜在的,更具危险性的,因而更值得引起广泛的注意。近年来,随着自由基生物学研究的日渐深入,已证实自由基所致的生化紊乱和组织结构破坏可作为许多疾病的发病学基础,故自由基在生物医学中的意义日益受到重视。过氧化脂质(LPO)是脂质和氧发生过氧化反应的产物,脂质过氧化反应过程本身也是产生和释放大量自由基的来源之一。人体内过量的LPO及丙二醛(MDA)和共轭二烯(CD)等毒副代谢产物可猛烈攻击DNA、蛋白质、糖酯、酶蛋白、多不饱和脂肪酸(PUFAs)和细胞生物膜等,致脂质过氧化损伤而诱发和加剧各种疾病。维生素C、维生素E、Beta-胡萝卜素更多还原

【Abstract】 Objective: To investigate the change of serum malondialdehyde(MDA), superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px) in a level of acute omethoate intoxicated rats and the ameliorating effects of vitamins C.Methods:1. 72 rats were equally assigned to the control group, 0.1 LD50 omethoate group, 0.5 LD50 omethoate group, and 0.5 LD50 omethoate +VitC(200mg/kg) group.2. Sampling and storage: The blood sample were obtained from the heart of the rats at 12h, 24h, and 48h after subcutaneous injection of omethoate. They were centrifuged at 3000 rpm for 10min and stored at -70° C until use. The serum Ache, MDA ,SOD and GSH-Px were determined .3. Atistical analysis: All adats were presented as means and standard deviations. They were compared using analysis of q test by SPSS-PC. A P value of <0.05 was considered significant.Results:1. Serum MDA level increased significantly in 0.1 LD50 and 0.5 LD50 omethoate group compared to the control group (P < 0.05), and increased significantly in 0.5 LD50 group omethoate compared to 0.1 LD50 omethoate group (P<0.05) .but decreased significantly in 0.5 LD50 omethoate +VitC group (P < 0.05).2. The activity of SOD and GSH-Px decreased significantly in 0.1 LD50 0.5 LD50 omethoate group compared to the control group (P < 0.05), decreased significantly in 0.5 LD50 omethoate group compared to 0.1 LD omethoate group (P<0.05).3. The activity of SOD and GSH-Px increased significantly in 0.5 LD50 omethoate +VitC group compared to 0.5 LD50 group omethoate (P < 0.05).conclusion:1. There are increase of lipid peroxidation in acute omethoate intoxicated rats.2. There are decrease of anti-oxidant enzyme activities in acute omethoate intoxicated rats.3. Treatment with vitamins C can reduce lipid peroxidation caused by omethoate.更多还原