【作者】 刘咏；

【导师】 罗建平；

【作者基本信息】 合肥工业大学 ， 农产品加工及贮藏工程， 2005， 硕士

【摘要】 霍山石斛（Dendrobium Huoshanenses G.Z.Tang et S.J.Cheng）是珍稀名贵中草药,目前已经濒危。为采用大规模反应器生产霍山石斛替代品,实现霍山石斛资源的可持续利用,本文对液体培养霍山石斛类原球茎快速增殖和多糖合成的条件进行了研究,并对类原球茎的保健功效进行了评价。 1.液体条件下霍山石斛类原球茎最适宜的基本培养基强度是1/2MS;通过单因素和正交试验,优化出类原球茎快速生长的增殖培养基是1/2MS+蔗糖30gL^-1+马铃薯水提液100gL^-1+香蕉水提液30gL^-1+IBA1.0mgL^-1,该培养基可使类原球茎在40d内增殖12.56倍。 2.葡萄糖和蔗糖对类原球茎生长与胞内多糖合成的实验结果表明,培养基中添加蔗糖有利于多糖合成,类原球茎多糖积累的优化培养基是1/2MS+马铃薯水提液150gL^-1+香蕉水提液30gL^-1+蔗糖30gL^-1+,此时类原球茎的多糖含量达9.78m-/g。对多糖合成与类原球茎生长采用Pearson相关分析,结果表明,二者是负相关的,相关系数为-0.845。 3.实验证明霍山石斛多糖具有明显的缓解视疲劳和增强免疫低下小鼠的机体免疫功能。霍山石斛类原球茎与茶叶按照7∶3（w/w）比例配制的保健饮品,不仅可使小鼠眼球和血清SOD活力分别提高1.64倍和1.47倍,而且能有效提高免疫低下小鼠的免疫器官指数,与环磷酰胺组比较,该饮品可分别使小鼠脾脏指数提高21.6%、胸腺指数提高79%。更多还原

【Abstract】 Dendrobium huoshanense GZ.Tang et S. J. Cheng is a rare precious Chinese herb. It has been in severe danger. In order to realize its sustainable utilization by mass propagation of protocorm-like bodies （PLBs） in bioreactor, culture conditions influencing proliferation and polysaccharide accumulation of PLBs were investigated in this paper. Furthermore, the health care efficacy of PLBs was evaluated .1) The most suitable basal medium for proliferation of PLBs in liquid culture system was basal MS at half-strength. Through one-factor and orthogonal tests, the optimum organic additives and plant regulators was obtained. The optimized medium, where the proliferation multiple of PLBs reached 12.56 after 40 days of culture, was 1/2MS supplemented with 30 g L^-1 sucrose, 100 g L^-1 potato extract, 30 g L^-1 banana extract and 1.0 mg L^-1 IBA.2) The regulation for growth and polysaccharide synthesis of PLBs by adding glucose and sucrose to medium respectively showed that sucrose responded better for polysaccharide accumulation. MS medium at half-strength containing 150 g L^-1 potato extract, 30 g L^-1 banana extract, 30 g L^-1 sucrose was suitable for polysaccharide accumulation and 9.78 mg polysaccharides per gram PLBs was obtained within 30 days. There is a negative correlation between polysaccharide accumulation and growth of PLBs by Pearson’s correlation analysis, the correlation coefficient was -0.845.3) The activity of polysaccharides from D. huoshanense PLBs that lessen eye tiredness and improve immune function in mice treated with cyclophosphamide （CY） was evaluated. Experiments showed that when the beverage prepared by mixing D. huoshanense PLBs and tea in 7:3 （w/w） ratio was given to mice, the activity of SOD in eyes and blood serum were significantly enhanced 1.64 times and 1.47 times and the organ indexes were improved. Compared to CY group, the spleen index and thymus index of the beverage group were 21.6% and 79.0% respectively.更多还原