【作者】 芮飞龙；

【导师】 郭层城；

【作者基本信息】 西北师范大学 ， 体育教育训练学， 2005， 硕士

【摘要】 本实验研究目的是通过探寻运动及中药复方宽筋散对闭合性软组织损伤急性期修复的部分作用机理,以期寻找快速、简洁、高效的、能够促进急性软组织损伤修复的方法和手段,减少组织损伤后修复过程中组织粘连及瘢痕增生的形成,尽最大程度改善预后。 针对闭合性软组织损伤后的红(rubor)、肿(tumor)、热(color)、痛(dolor)及功能障碍(functiolaesa)的典型临床体征,加上无骨折、无皮肤破损的特点,我们设计了相应的实验,即热板法致小鼠疼痛的痛阈实验;福氏完全佐剂(Freun’s complete adjuvant,FCA)致大鼠足肿(抗炎)实验;二甲苯致小鼠耳肿(消肿)实验;用血流变仪测血液流变性的活血化瘀实验;运用高效液相色谱—电化学(high performance liquid chromatography-electrochemistry Detection HPLC-ECD)法测量大脑及血清中单胺类神经递质的实验以及免疫组织化学法(immunohistochemistry)检测原癌基因C-FOS蛋白产物表达的实验。从分子生物学水平及基因水平上探讨运动加宽筋散在闭合性损伤急性期修复的的作用机制。结果显示: 1 急性闭合性软组织损伤模型复制成功。本实验以重锤打击法制造急性闭合性软组织损伤模型,较好地再现了闭合性软组织损伤后的病理过程,为进一步比较实验奠定了基础。 2 适量运动加宽筋散能显著提高热板法所致小鼠的痛阈。 3 适量运动加宽筋散能抑制二甲苯致小鼠耳廓肿胀和福氏完全佐剂所致大鼠足趾肿胀,具有较好的消肿抗炎作用。 4 适量运动加宽筋散可以显著改善软组织损伤模型动物的血液流变性,具有良好的活血化瘀功效。 5 适量运动加宽筋散能促进软组织损伤模型小鼠大脑中单胺类神经递质的释放(抑制NE的释放),从分子水平上揭示其有一定的镇痛功效。 6 适量运动加宽筋散可以明显降低软组织损伤模型大鼠血清中单胺类神经递质的含量,减少了组织损伤后内源性刺激物的释放,有利于损伤组织的修复。 7 适量运动加宽筋散可有效抑制软组织损伤模型大鼠中枢及外周C-FOS基因表达,从基因水平揭示其具有很好的镇痛作用。更多还原

【Abstract】 This experiment aims at looking for some faster,simpler,and more efficient means on fasting acute repairing of soft tissues damaged,reducing the tissues’ adhesion and preventing the hyperplasia of scar.and improving the prognosis to the greatest extent by seeking some mechanism in acute repairing period of exercise and traditional Chinese medicine edicine Kuanjingsan for closing soft tissue damaged.For it is rubor after damaging to the closing soft tissue, getting swollen, getting hot aching and model of functiolaesa these bed solicit and there is no fracture,no skin damaged, we designed the corresponding experiment, namely the law of hot board causes little mouse’s painful experiment of threshold of pain; Freun’s complete adjuvant(FCA) caused the swollen experiment of big mouse’s foot (resist the inflammation);The xylene caused the swollen experiment of little mouse’s ear (subside a swelling ); Invigorating blood circulation silt experiment that changes the sex that the rheology appearance examines the blood and flows with the blood; Useing the high performance liquid chromatography-electrochemistry Detected (HPLC-ECD) monoamine neurotransmitters transmitter in the brain and serum; the experiment of immunohistochemistry measured the proto-oncogene C-FOS expresses. On the basise probing into the function mechanism that exercise and Kuanjingsan break up on molecular biology level and gene level repaired in acute closing damage and doing the toxic acute toxicity experiment which was designed for verifying the toxicity of Kuanjingsan,we got the following result.1 Useing model got successed: we used heavy hammer to hit to make acute closing soft tissues damaging model,which can show the pathology course very well after orgonization’s damage as well as establishing the foundation for relatively testing further.2 Fitting exercise and Kuanjingsan can notably improve the threshold of pain of the little mouse that hot board caused.3 Fitting exercise and Kuanjingsan can suppress the xyol to cause little mouse’s auricle swelling and good fortune to totally assist the big mouse’s digit swelling that thepharmaceutical cause , there is better subsidence of a swelling that resists inflammation function.4 Fitting exercise and Kuanjingsan can improve the hemorheology of the model animal whose soft tissues were damaged and they have good function of invigorating blood circulation silt.5 Fitting exercise and Kuanjingsan can promote the soft tissue to damage little mouse’s release of the single amine neurotransmitter in the brain of models,and it announces that there is a certain efficiency of easing pain in it from molecule level.6 Fitting exercise and Kuanjingsan can reduce soft tissue damage model old mouse single amine content of neurotransmitter in the serum obviously to come loose, the endogenous release which stimulates the thing, help to damage the repair organizing after reducing and organizing and damaging.7 Fitting exercise and Kuanjingsan can suppress the soft tissue to damage big mouse’s centre of models and periphery C-FOS gene expression effectively, which announces that they can ease pain very well from the gene level.更多还原