【作者】 王生；

【导师】 四荣联； 李玉平；

【作者基本信息】 河北医科大学 ， 皮肤病与性病学， 2005， 硕士

【摘要】 目的: 微小染色体维持蛋白-2 (Minichromosome maintenance protein 2, MCM-2 protein)在真核细胞DNA 复制中起着调节DNA解旋酶的重要作用,仅在增生细胞中表达,是一种新的反映细胞增生情况的标记物。目前国内尚无检测MCM-2 蛋白在皮肤中表达的实验研究。本实验通过免疫组化的方法检测MCM-2 蛋白在不同部位的正常皮肤、皮肤良性增生、恶性增生病理改变皮损的表达,以了解MCM-2 蛋白在正常皮肤、良性增生病理改变皮损、恶性增生病理改变皮损中表达的强度及分布特点。通过该研究以探索MCM-2 蛋白能否成为指导皮肤科医师诊断各种良性和恶性增生性皮肤病的一个有价值的指标之一。MCM-2 蛋白是复制前复合物的一员,在真核细胞DNA复制中起着调节DNA 解旋酶的重要作用,保证DNA 在每个细胞复制周期中仅复制一次,并且仅在增生扩散的细胞中表达。因而,MCM-2 蛋白可以作为一种精确的增生细胞标记物。方法:选择15 例病理诊断为鲍温氏病或高分化鳞状细胞癌(I 级或II 级)患者皮损作为皮损I 组,其中鲍温氏病6 例,鳞状细胞癌9 例。29 例病理改变为假上皮瘤样增生或乳头瘤样增生的患者(病理诊断:着色真菌病4 例,孢子丝菌病2例,脂溢性角化5 例,寻常疣4 例,慢性湿疹4 例,皮肤纤更多还原

【Abstract】 Objective : The MCM-2 ( Minichromosome maintenance-2 protein) plays an important role that adjust DNA unwindase in replication of eucaryotic DNA . It only expresses in proliferated cells. It is a novel marker of proliferated cells . Now there is not domestic study about the expression of the MCM-2 in skin.This study detected the expression of the MCM-2 in different regional normal skin,the lesions with benign and malignant hyperplasia by immunohistochemical SP method . To explore whether MCM-2 could be one of the valuable markers for dermatologists to diagnose various kinds of benign and malignant hyperplasia skin disease. MCM-2 was a component of prereplication compound . It playes a very important role in adjusting DNA untwisting enzyme in eucaryotic cell , ensured DNA replication only once in every cell replication cycle, and only expressed in proliferative cells . As a result, MCM-2 could act as a precise marker of proliferative cell . Method: The lesion group I included fifteen patients with Bowen’s disease or well-differentiated Squamous cell carcinoma (I class or II class), they were finally diagnosed pathologically. There were six patients with Bowen’s disease , and nine patients with well-differentiated Squamous cell carcinoma. The lesion group II included twenty-nine patients with Pseudoepitheliomatous hyperplasia or papillomatous hyperplasia ( finally diagnosed pathologically: chromomycosis 4 cases, sporotrichosis 2 cases, seborrheic keratosis 5 cases,verruca vulgaris 4 cases, chronic eczema 4 cases, dermatofibroma 4 cases, non-specificity dermatitis 6 cases). The normal control group included 37 healthy adult’s skin. The samples were taken from face ( 7 cases), limbs( 9 cases ),armpit( 8 cases ),trunk( 8 cases),metacarpus or metatarsus ( 5 cases ). All lesion samples were paraffin block file from department of dermatology,second hospital of Hebei medical college, from January 1998 to June 2004 , and they were all made definite pathological diagnosis. All normal samples were superfluous skin obtained by operation and were followed the standard processing to make paraffin sections. The distribution and intensity of expression of MCM-2 in the epidermis of the samples from well-differentiated Bowen’s disease or Squamous cell carcinoma(lesion group I), Pseudoepitheliomatous hyperplasia or papillomatous hyperplasia(lesion group II) and health adult (normal control group) were detected by immunohistochemical SP method . Results1.The distribution of sex and age: In the lesion group I , male 10 patients(66.67%) and female 5 patients(33.33%) , the oldest patient was 80 years and the youngest was 40 years, average age was 66.76±15.36 years. In the lesion group II , male 17 patients(58.62%) and female 12 patients(41.38%) , the oldest patient was 78 years and the youngest was 30 years, average age was 66.25±14.37 years. In the normal control group, male 23 patients(62.16%) and female 14 patients(37.84%), the oldest patient was 70 years and the youngest was 55 years, average age was 65.52±16.65 years. 2.The part of MCM-2 expression: The distribution of MCM-2 positive cells in lesion group I ,lesion group II and normal control group was different. In the lesion group I , MCM-2 expressed not only in the cells of stratum basalum but also in the cells of stratum spinosum and above it. In the lesion group II MCM-2 expressed in the cells of stratum basalum and in the a small quantity cells of stratum spinosum. In the control group, MCM-2 expressed only in stratum basalum’cells. 3.The intensity of the MCM-2 expression in the epidermis: The intensity of the MCM-2 expressed in the epidermis of the samples from the lesion group I ,lesion group II and normal control group was different. In the lesion group I : significantly positive lesion 8, positive lesion 5, weakly positive lesion 1, negtive lesion 1; In the lesion group II : significantly positive lesion 3 ,positive lesion 18, weakly positive lesion 6,negtive lesion 2; In the normal control group: positive lesion 1, weaklypositive lesion 30, negtive lesion 6. Af更多还原