【作者】 周梅；

【导师】 黎音；

【作者基本信息】 新疆医科大学 ， 病理， 2004， 硕士

【摘要】 目的:研究cyclin E、p21^WAF1/CIP1和MMP-2蛋白在甲状腺癌中的表达情况,及其与甲状腺癌发生、发展的关系。方法:采用免疫组织化学LSAB法对47例甲状腺癌进行免疫组化检测,取17例甲状腺腺瘤、14例结节性甲状腺肿及12例正常甲状腺组织作对照。结果:（1）在正常甲状腺组织、结节性甲状腺肿、甲状腺腺瘤和甲状腺癌中,p21^WAF1/CIP1蛋白的阳性表达率分别为0%（0/12）、7.1%（1/14）、29.4%（5/17）、78.7%（37/47）;cyclin E蛋白的阳性表达率分别为0%（0/12）、14.3%（2/14）、47.1%（8/17）、87.2%（41/47）;MMP-2蛋白的阳性表达率分别为0%（0/12）、7.1%（1/14）、23.5%（4/17）、78.7%（37/47）。三种蛋白在甲状腺癌中的阳性表达率均显著高于其它对照组（P<0.05）。（2）MMP-2在不同组织类型甲状腺癌中的表达差异有显著性（P<0.05）;（3）MMP-2在有淋巴结转移的甲状腺癌中高表达,MMP-2的表达与淋巴结转移显著相关（P<0.05）;（4）在甲状腺中cyclin E与p21^WAF1/CIP1、cyclin E与MMP-2蛋白表达无相关性（P>0.05）。结论:（1）p21^WAF1/CIP1、cyclin E及MMP-2蛋白表达可能在甲状腺癌的发生发展中起重要作用;（2）联合检测cyclin E、p21^WAF1/CIP1和MMP-2蛋白的表达将有助于良恶性甲状腺肿瘤的鉴别及预后的判断。更多还原

【Abstract】 Objective: To investigate the expression of cyclin E, p2lWAF1/CIP1 and MMP-2 in thyroid carcinoma and its relation to carcinogenesis of thyroid carcinoma. Methods: The expression of cyclin E, p21WAF1/CIP1 and MMP-2 were studied in 47 cases of thyroid carcinoma by immunohistochemical technology . 17 cases of thyroid adenoma, 14 cases of nodular goitre and 12 cases of normal thyroid tissue served as control. Results: (l)In normal thyroid tissue, nodular goitre, thyroid adenoma and thyroid carcinoma, the positive rate of cyclin E protein was 0% (0/12) , 14.3% (2/14) , 47.1% (8/17) , 87.2% (41/47) respectively; p21WAF1/CIP1 protein was 0%(0/12) , 7.1% (1/14) , 29.4% (5/17) , 78.7% (37/47) respectively ; MMP-2 protein was 0% ( 0/12 ) , 7. 1%(1/14) , 23.5% (4/17) , 78.7% (37/47) respectively. The positive rate of three proteins in thyroid carcinoma was notably higher than normal thyroid, nodular goiter and thyroid adenoma( P <0.05). (2) The expression of MMP-2 was correlated with different histologic types of thyroid carcinoma(P <0. 05) ; (3) The expression of MMP-2 was high in thyroid carcinoma with lymph node metastasis, The frequency of overexpression of MMP-2 was associated with lymph node metastasis(P <0.05); (4)There was not a significant correlation betweencyclin E and p21WAF1/CIP1 , cyclin E and MMP-2 protein (P >0. 05). Conclusion: (1) The expression of p21WAF1/CIP1, cyclin E and MMP-2 may play an important role in the development of thyroid carcrinoma; (2)The detection of p21WAF1/CIP1, cyclin E and MMp-2 is valuable todifferentiate between benign and malignant neoplasm of thyroid gland and prognsis.更多还原