【作者】 王松；

【导师】 徐晏； 丁剑冰；

【作者基本信息】 新疆医科大学 ， 免疫学， 2003， 硕士

【摘要】 目的:通过观察经实验诱发细粒棘球蚴病小鼠体内四种细胞因子水平的变化,研究棘球蚴病的免疫学发病机制。方法:小鼠腹腔接种细粒棘球蚴原头节,持续观察220天。收集血清用ELISA法检测,研究其血清中IL-2、IFN-γ、IL-4、GM-CSF水平的变化。结果:与正常对照比较,IL-2在感染的初期除第1天有所升高外,其余时间均低于正常值,100天后呈波动型上升,而在220天达到峰值。IFN-γ在感染的前100天均在正常值以下波动,后急剧升高,至120达到峰值,140天后又急剧下降。IL-4在140天前呈增高趋势,以后逐渐下降。GM-CSF始终在正常水平以上波动,在第一天即接近峰值,其中在100天达到峰值以后逐渐下降。结论:机体抗包虫免疫中的一个重要机制是Th2辅助的体液免疫与Th1介导的细胞免疫共同作用的结果。Th1在感染100天后出现优势活化,而Th2在感染后140天出现优势活化,此外,感染中非特异性免疫的作用也不容忽视。更多还原

【Abstract】 Objective: We have investigated pathogenesis of immunology in mice with experimentally induced secondary Echinococcus Granulosus (Eg) after detected the variable levels of four cytokines. Methods: Following abdominal inoculation of echinococcus protoscoleces, we research the change of serum level of IL-2, IFN-r, IL-4 and GM-CSF with ELISA method. The course of the infection was followed for 220 days. Results: As compared with uninfected controls, the level of IL-2 showed U-shape after 100 days postinfection (p.i.) below the value of control group, then had a fluctuant increase, it showed a peak at 220 day p.i.. The level of IFN-r was lower than normal before 100 day p.i, the peak value occurred at 120th day p.i., whereas it sharply decreased after 140 days p.i.. The level of IL-4 was significantly increased during the first 140 days, and then there was a slow decrease after 140 day post infection. During the course of infection, higher level of GM-CSF were detected than normal, and it showed a peak at 100 day post infection. Conclusions: The data suggest the induction of Th2 AMI with a parallel expansion of Thl CMI as important mechanism of host defence against echinococcus. Two subtypes of Th cell occur activation one after another, Thl is earlier than Th2, in addition, the non-specific immunity is important in EG infection.更多还原