【作者】 贺长水；

【导师】 周真；

【作者基本信息】 哈尔滨理工大学 ， 精密仪器及机械， 2004， 硕士

【摘要】 生物传感器作为一种新型的生物信息技术已经在生物医学领域发挥了重要作用,用于检测苯丙酮尿症的苯丙氨酸解氨酶(PAL)的酶电极具有重要的研究意义和广泛的应用前景。本文研究的苯丙氨酸浓度测试系统,由信号采集模块、信号处理模块、控制运算模块和人机模块组成。以苯丙氨酸解氨酶电极为核心的信号采集模块,通过固定化生物活性酶和氨气敏电极完成由非电量导电量的转换过程。在对课题进行综合分析和前期的工作总结基础上,本文完成了以下研究:根据酶电极信号微弱、易受干扰特性进行硬件设计和硬件可靠性设计。根据临床应用的特点进行测试系统软件设计和操作触摸屏设计,达到界面友好、易于操作、可靠性高的要求。对测试系统数据分析和线性化校准进行研究,提供最优化数据处理方案和算法。对系统进行误差分析,建立系统误差数学模型。此项目研究的意义:实现提早对PKU进行定量检测,对减轻家庭和社会负担,提高人口素质具有不可估量的意义。鉴于苯丙氨酸在营养学、医学等领域有重要作用,苯丙氨酸浓度的检测对于苯丙氨酸生产、利用等方面具有关键意义。由于此方法基于新型酶电极,对于酶生物传感器发展有很高学术价值。所开发的检测系统应用于苯丙酮尿症的临床检验,克服了以往方法检测周期长、成本高、误诊率高的缺点,但仍有问题存在,比如酶电极的重复性需要进一步提高,酶的修饰和固定化技术需要进一步研究等。更多还原

【Abstract】 Being a new biologic information technics, biosensor exerts a profound influence on the biomedicine domain. The bioelectrode constituted by phenylalanine ammonia-lyase (PAL) was used in PKU diagnosis, which has significant academic value and extensive applying field.The detecting system is composed of signal sample module, signal-processing module, controlling module and human-machine module. Being the core of signal sample module, the bioelectrode converts nonelectric signal to electric signal by PAL and ammonia electrode. Based on the analyse of the task and the summarize of prophase study, the main contents are stated as followed:Hardware design has been finished according to characters of the PAL bioelectrode signal. The signal is faint and easy to be disturbed.Control software and tuch screen software have been desiged according to clinical characters. The design satisfies the request of high reliability in clinic application. The human-machine screen is friendly and easily in operation.Based on detecting datum of the system, the thesis gives method and algorithm for optimizing. The thesis gives error analysis and error mathematical model.The study meaning is stated as followed:The system Realies PKU diagnosis as early as possible, it has more social meaning.Viewing for the importance of PAL in nutriology and medicine, the research has the key meaning of PAL’s production and application.The research is very valuable for the enzyme bioelectrode development.The system overcomes shortcomings in former method, such as long diagnostic period, high cost and misdiagnoses. It has problems also, such as the bioelectrode’s repeatability, reliability and fastness technics of enzyme needs to study further.更多还原