【作者】 戚纪胜；

【导师】 吴光；

【作者基本信息】 延边大学 ， 内科学， 2004， 硕士

【摘要】 目的：通过观察大鼠海马区突触素和GAP-43表达的变化，探讨长期饮酒及辅酶Q₁₀对其的影响。 方法：将60只三月龄大鼠随机分为对照组12只、高剂量酒精（HA）组24只、低剂量酒精（LA）组24只，饲养5周后，再随机将高剂量酒精组分为HA1组、HA2(高剂量酒精+辅酶Q₁₀)组，将低剂量酒精组分为LA1组、LA2(低剂量酒精+辅酶Q₁₀)组，再饲养5周后，用免疫组化方法和图像分析技术，检测突触素及GAP-43在海马区的表达。 结果：LA1组及HA1组突触素免疫反应产物校正光密度（COD）值均较对照组显著降低（P＜0.01），HA1组较LA1组明显降低（P＜0.05）。LA2组较LA1组明显增高（P＜0.05），HA2组与HA1组间无明显差异（P＞0.05）。LA2组GAP-43免疫反应产物校正光密度（COD）值较对照组、LA1组明显增高（P＜0.05），HA1组与LA1组、HA2组与HA1组间无明显差异（P＞0.05）。 结论：长期饮酒可引起大鼠海马突触素含量下降，其下降程度与饮酒量有关。辅酶Q₁₀可能对低剂量长期饮酒造成的中枢神经损伤有治疗作用。更多还原

【Abstract】 Abstract Through observing the expression of synaptophysin and GAP-43 in rat hippocampus ,to investigate the effects of long-term Alcohol consumption and CoQ10 .Methods 60 male Wistar rats were divided into 3 random groups : 12 for normal control (N), 24 rats were orally exposed to high dosage of alcohol (HA, 6mg/kg/d), Low dosage of alcohol was given to the other 24 rats (LA, 2mg/kg/d). 5 weeks later, HA was randomized into HA1(continue with alcohol) and HA2(alcohol and CoQ10), so as LA.After all 10 weeks, a quantitative evaluation of synaptophysin and GAP-43 was performed in hippocampus by using immunohistochemical method and the results were processed by computer image analysis system.Results The immumoreactive intensity of synaptophysin declined in hippocampus of LA1 and the HA1 ,which had significant difference compared with the control group(p<0.01).There were obvious difference between LA1 and HA1(p<0.05).The difference was also notable between LA1 and LA2(p<0.05).The immunoreactivities of GAP-43 in hippocampus of LA2 was stronger than the control group and LA1(p<0.05).Conclusion Long-term alcohol consumption lead to the decrease of synaptophysin and the decrease-degree is related with the quantity of alcohol consumption.CoQ10 presumably have a curative action on the central nerve system damage induced by long-term Low-dosage-alcohol consumption.更多还原