【作者】 邱昌桂；

【导师】 任凤莲；

【作者基本信息】 中南大学 ， 分析化学， 2004， 硕士

【摘要】 本文用正交试验法探讨了百合中总皂甙的最佳提取工艺条件，确定了百合中总皂甙的最佳提取工艺条件即：提取温度（A）为70℃，乙醇浓度（B）为80％，固液比例（C）为1：6，提取时间（D）3h，提取次数（E）3次。五个因素的影响大小次序为E＞A＞D＞C＞B。在此条件下提取，测得百合中总皂甙的含量为3.48mg／g。 本文建立了百合中总皂甙的定量测定方法。以薯蓣皂甙为对照品，香草醛-高氯酸为显色剂，冰醋酸为溶剂，60℃恒温15min，分光光度法测定百合总皂甙的含量。最大吸收波长为540nm，浓度与吸光度线性关系良好，相关系数r=0.9996。用加标回收法测定样品，回收率在96.74％～103.65％之间，RSD=3.0％。该方法灵敏度高、重现性好。 通过对X-5、AB-8和NAK-9型三种大孔吸附树脂对百合总皂甙的吸附性能研究，筛选出适宜分离百合总皂甙的树脂为AB-8型。并研究了利用AB-8型大孔吸附树脂纯化处理百合提取液的工艺条件，确定AB-8型树脂分离皂甙的最佳洗脱剂为70％乙醇溶液。 探讨了百合提取物对羟自由基的清除能力。以溴邻苯三酚红（BPR）为显色剂，基于Co（Ⅱ）-H₂O₂体系反应产生的羟自由基（·OH）使BPR的颜色发生变化，采用756型紫外-可见分光光度计测定其吸光度的变化，可间接测定·OH的方法。并对百合提取物的羟自由基清除能力进行评价，得出提纯的百合提取物对羟自由基清除能力高于百合的粗提物。更多还原

【Abstract】 The technology of extracting total saponins from the bulbs of lilium brownii was studied .The orthogonal design was used to optimize the extraction process of lilium brownii with content of total saponins. The result showed that the optimum extraction conditions obtained were temperature 70 C, 80% alcohol, solid/liquid of 1 : 6 and reflux time of 3 h with 3 times. The order of these five factors on affecting the extracting yield of total saponins was: E>A>D>C>B. In the condition,the concentration of total saponins was 3.48%.An accurate method for determination of the content of total saponins in lilium brownii was reported. The dioscin was used as the control sample, the vanillin-perchloric acid as chromogenic agent, glacial acetic acid as solvent system at 60Cfor 15min.Then,the content of total saponins in lilium brownii were determined by spectrophotometry at 540nm.The calibration curve had good linearity, r=0.9996. The recovery ratio of measurement was 96.74%~ 103.65%, and the relative standard deviation was 3.0%. The method had good sensitivity and reappearance.Three kinds of macro porous adsorbents, "X-5, AB-8 and NAK-9 were studied on adsorption performance for total saponins of lilium. It was showed that AB-8 was a good adsorption resin for total saponins of lilium. Technological process of purification was studied with the macro porous adsorbent of AB-8. The optimum conditions were: after extracting coarse solution of saponins, adsorption and separation by AB-8 resin with 70% ethanol, which was used as eluted solvent.The study was performed to investigate the radical scavenging activity of extracting of lilium. Bromopyrogallol red(BPR) was used as chromogenic reagent. A system Co2+~H2O2 produces hydroxyl radical ( OH) by reaction between Co2+ and H2O2 while the hydroxyl radical change the color of BPR. The amount of -OH can be indirectly assayed through absorbance change by means of UV-Vis spectrophotometer. The Hydroxyl radical scavenge activity of extracting of lilium was evaluated. The results showed the purify extract of lilium had higher ability toscavenge hydro-radical than that of the coarse extract of lilium.更多还原