【作者】 姜晓东；

【导师】 马泽芳； 李和平；

【作者基本信息】 东北林业大学 ， 野生动植物保护与利用， 2004， 硕士

【摘要】 本研究采用放射免疫分析方法（RIA），对雌性梅花鹿妊娠早期外周血液中五种生殖激素（促黄体素LH、促卵泡素FSH、雌二醇E₂、孕酮P和人绒毛膜促性腺激素hCG）的含量变化进行测定。 试验于2002～2003年在吉林松花湖实验林场养鹿场进行。试验中所选鹿只均为体况良好、健康无疾病的经产梅花鹿。采集血样时对鹿只采用半自动夹板式保定装置（吊圈）保定的方式，于其眶下静脉进行采集。 试验分A、B两组进行。A组中选择6头雌性梅花鹿，采用试情的方法，在揭示其发情后对3头进行配种，剩下3头梅花鹿不参加配种，分3次采集血样；B组选择4头妊娠的雌性梅花鹿，对妊娠早期（2～38d）进行血样采集，对比LH、FSH、E₂、P和hCG在未妊娠与妊娠早期外周血液中的含量变化。研究结果表明： 1、未妊娠与妊娠的雌性梅花鹿在发情后2-6d内LH、FSH、P和hCG的含量均无显著性变化（p＞0.05）。 2、未妊娠的雌性梅花鹿E₂含量在发情后第4d（3.75±0.49pg／ml）与第6d（3.75±0.35pg／ml）分别显著高于第2d（2.32±0.19pg／ml）（p＜0.05），而第4d与第6d相比差异不显著（p＞0.05）。 3、妊娠的雌性梅花鹿其LH、E₂和hCG的含量在妊娠后的38d内均无明显变化（P＞0.05）。 4、FSH的含量除了在其妊娠的第14d与第30d和38d相比有显著差异（p＜0.05）及妊娠第20d与第24d、30d和38d相比有显著差异（p＜0.05）外，均无明显变化（p＞0.05）。 5、从妊娠开始，随着时间的推移P的含量逐渐增加。第14d出现第一个阶段的高峰（0.08±0.01ng／ml）后开始缓慢降低；至第26d出现一个低峰（0.01±0.01ng／ml）；随后又迅速上升，在第34d出现最高峰值（0.14±0.037ng／ml）（p＜0.01）。 6、妊娠第34d P所出现的最高峰值（p＜0.01），可作为梅花鹿早期妊娠诊断的观测点。 通过对东北梅花鹿妊娠早期外周血中五种生殖激素含量的比较研究，进而为梅花鹿生殖内分泌的基础性研究以及梅花鹿的早期妊娠诊断、发情鉴定等繁殖技术方面的研究提供了理论依据。更多还原

【Abstract】 It was radioimmunoassay (RIA) that we used to assay the five primary reproductive hormones (luteinazing hormone-LH, follicle stimulating hormone-FSH, estradiol-E2, progesterone-P and human chorionic gonadotrophin-hCG) levels in peripheral serum of the female Sika deer during estrus and early pregnancy.This experiment was performed from 2002 to 2003 at the Deer Farm of Songhua Lake’s Experimental Forestry Farm. All the deer which we selected had every produced fawns and were fire and healthy. The serum samples were drawn from the veins below eye sockets of the deer, which had been fastened by the semiautomatic clipping-boarded device.This experiment was partitioned into two parts, that is, Team A and Team B. There were six female Sika deer chosen in Team A. After being confirmed that they had all been in heat, they were separated that one half of them were made to copulate with the breeding deer and the other half were not to be comparison. The serum samples of these six deer were respectively collected for three times. In Team B there were four female Sika deer pregnant all, from which we obtained serum samples to analyze the five reproductive hormones’ concentration and the variation change during the early pregnancy (2~38d). The results of the studies are as followings:1.The levels of LH, FSH, P and hCG of the estrum and pregnancy female deer during the period 2nd~6th days after the deer had been in heat did not show and significant change (P>0.05).2. The estradiol concentration on the 4th day and the 6th day after estrum were all significantly higher than that on the 2nd day (P<0.05). But they didn’t show any significant change to each other (P>0.05).3. The pregnancy female deer’s LH, E2, and hCG levels also showed no significant variation (P>0.05).4. FSH levels showed two significant changes (P<0.05). One was occurred on the 14th day after pregnancy compared with the 30th and 38th day.5. Form the beginning of pregnancy, the concentration of progesterone was gradually increasing with the time went by. After appearing a high peak of the first phase on the 14th day, the concentration began to reduce until the 26th day, when showed a lowpeak. Then the levels rose rapidly. There appeared the highest peak on the 34th day6. The concentration on the 34th day after pregnancy can be the optimum serum collecting time for the early pregnancy diagnosis.What we studies on the five primary reproductive hormones levels in peripheral of the female Sika deer during the early pregnancy would be serviced for the essential studies on the reproductive endocrinology of Sika deer and the early diagnosis of pregnancy, the appraisal of estrus and the other reproductive technologies.更多还原