【作者】 晏国全；

【导师】 张寒琦；

【作者基本信息】 吉林大学 ， 分析化学， 2004， 硕士

【摘要】 利用微波辅助萃取法(MAE)萃取刺五加中的黄酮类(金丝桃苷、芦丁、槲皮苷、槲皮素)化合物和皂苷类化合物。应用高效液相色谱法测定了其中的黄酮类化合物。并通过正交实验，考察了微波萃取条件(包括溶剂、微波辐射时间、萃取压力和料液比)对刺五加中总皂苷和总黄酮萃取率的影响规律。实验结果表明，溶剂为50％乙醇、萃取压力为700kPa、萃取时间为10min、料液比为1∶20时，总黄酮萃取率最佳。与索氏萃取法相比，萃取率提高了40％。溶剂为70％乙醇、萃取压力为700kPa、萃取时间为10分钟、料液比为1∶30时，总皂苷萃取效率最佳。与索氏萃取法相比，萃取率提高了34％。实验结果显示，与传统的索氏萃取方法相比，微波辅助萃取方法能够显著减少萃取时间和萃取溶剂用量，提高了萃取率和萃取选择性。微波辅助萃取法在中药有效成分萃取中具有良好的应用前景。更多还原

【Abstract】 Flavonoids (hyperin, rutin, quercitrin and quercetin) and saponins compounds from Acanthopanax Senticosus Harms have been extracted by means of a microwave-assisted extraction(MAE) technique. Flavonoids have been determined by high-performance liquid chromatography. The influence of some operation conditions, such as ethanol concentration, microwave irradiation time and processing pressure, on the extraction yield of those compounds has been studied by using orthogonal test and optimization based on multivariate statistics. The experimental results show that the highest extraction yield of flavonoids can be obtained when extraction solvent is 50% ethanol, processing pressure is 700kPa, microwave irradiation time is 10 minutes and the ratio of sample weight to solvent volume is 1:20. In the optimal conditions of MAE, the extraction yield offlavonoids is higher 40% than that with Soxhlet extraction. The highest extraction yield of saponins can be obtained when extraction solvent is 70% ethanol, processing pressure is 700kPa, microwave irradiation time is lOminutes and the ratio of sample weight to solvent volume is 1:30. In the optimal conditions of MAE, the extraction yield of saponins is higher 34% than that with Soxhlet extraction.The experimental results show that, comparing with Soxhlet extraction, MAE can drastically reduce the time of extraction and the amount of solvent consumption, improve extraction yield and selectivity. The MAE is a promising technology for extracting active compounds from Chinese material medica.更多还原