【作者】 张峰；

【导师】 甄二英； 张英杰；

【作者基本信息】 河北农业大学 ， 动物营养与饲料科学， 2004， 硕士

【摘要】 选用80只5月龄的杂交绵羊(萨福克×小尾寒羊)，随机分为四组，研究了不同氮源对绵羊生长性能、消化代谢及血清部分生化指标的影响。对照组饲喂A常规日粮，试验组分为三个组别，分别为尿素组饲喂B日粮(2％尿素)、包被尿素组饲喂C日粮(2％包被尿素)、组合组饲喂D日粮(2％包被尿素+0.2％益生素)。通过对杂交绵羊的生长性能、消化代谢、血清中的碱性磷酸酶(AKP)、谷丙转氨酶(GPT)、谷草转氨酶(GOT)、尿素氮(BUN)、血清pH值等指标的测定。结果表明：(1)B、C和D日粮与A日粮相比，总增重量和日增重差异不显著(P＞0.05)，但C、D日粮总增重和日增重要大于其他组，有增加的趋势，其中以B日粮增重最少；(2)C和D日粮可以使绵羊沉积氮与可消化氮的比例提高3.68％～17.56％，且显著减少绵羊尿氮的排出(P＜0.05)。和其他日粮相比，D日粮能显著提高绵羊的沉积氮。各组进食干物质、进食氮、粪氮、可消化氮之间差异不显著(P＞0.05)；(3)D日粮可以显著提高绵羊的干物质消化率、氮表观消化率、ADF和NDF的消化率以及酸不容灰分的利用率(P＜0.05)，其他各日粮之间差异不显著；(4)饲喂后两小时B、C、D日粮血清中碱性磷酸酶的活性都有不同程度的提高，与A日粮相比，差异显著(P＜0.05)，四小时后各日粮间血清碱性磷酸酶活性趋于一致；(5)各日粮血清中谷草转氨酶活性在饲喂后两小时开始升高，其中以B日粮最高，与A日粮相比，差异显著(P＜0.05)，其他各日粮差异不显著，随着时间的变化，各组血清中谷草转氨酶活性开始下降，其中以B日粮下降较慢，四小时以后各组血清中谷草转氨酶活性又降到正常水平；(6)血清中谷丙转氨酶活性各组都有升高，以B日粮升高最快。与A日粮相比，三个试验日粮差异显著(P＜0.05)，随后各日粮血清中谷丙转氨酶活性开始下降，四小时后，与A比较，C和D差异不显著(P＞0.05)；(7)饲喂后两小时，试验日粮血清中的尿素氮水平显著高于A日粮(P＜0.05)，四个小时后血清中尿素氮水平达到最高值，随着时间的变化，各日粮血清中尿素氮水平接近一致，且差异不显著(P＞0.05)，但三个试验日粮血清尿素氮要高于A日粮9％～36.4％；(8)饲喂后两小时B日粮血清pH值显著高于其他各日粮(P＜0.05)，其他日粮之间无显著变化(P＞0.05)，四小时后A日粮与试验日粮血清pH值差异不显著。本试验结果表明，绵羊日粮添加2％尿素、2％包被尿素、2％包被尿素+0.2％益生素安全可靠，效果明显，试验日粮对绵羊的血清生化指标均在正常范围之内，其中，以C、D日粮对氮利用率效果最好。更多还原

【Abstract】 In order to study the changes of performance, the digestion and metabolism and blood biochemical materials in sheep.eighty hybrid sheep(suffork X small-tailed nan sheep) of 5 months old were divided randomly into four groups. The control group was fed A(usual feed), the urea group, wrap urea group and combination group were supplied with B(2%urea), C(2%wrap urea), D(2%wrap urea+0.2%probiotic) diet respectively. The performance, the digestion and metabolism . the blood biochemical materials(AKP, GPT, GOT, BUN, pH) of sheep were measured respectively. The results as follows:(1) The tatal weight gain and average daily gain were not affected (P>0.05)when sheep were fed B, C and D diets compared with A diet, but the tatal weight gain and average daily gain was increased when the sheep fed C and D diet compared with other diets, the tatal weight gain of B diet was least than other diets.(2) The ratio deposit nitrogen and digestible nitrogen were increased by 3.68%-17.56% respectively, when sheep were fed C and D, but there were no difference(P>0.05). Urine nitrogen was decreased significantly(P<0.05) when sheep fed C and D diet. The deposit nitrogen was increased significantly(P<0.05) when sheep fed D diet compared with other diet.Among A, B. C and D diet, the dry matter, feces nitrogen, digestible nitrogen were no difference(P>0.05). (3)Dry matter digestion coefficient, nitrogen apparent digestion coefficient, ADF digestion coefficient, NDF digestion coefficient and AIA coefficient were increased significantly(P<0.05) when sheep fed D diet, among A. B and C diet, there were no difference(P>0.05).(4)the blood AKP was increased after 2 hours when sheep fed B. C and D dietthere were greater(P<0.05) compared with A diet, there were no difference(P>.05) after 4 hours among A, C and D diets. (5)Blood GOT was increased after 2 hour when sheep fed A, B> C and D diets,GOT was increased significantly(P<0.05) when sheep fed B diet compared with A diet, there were no difference(P>0.05) among A, B> C and D diets.(6)Blood GPT was increased when sheep fed A, B, C and D diets,GPT was increased quickly when sheep fed Bdiet,the blood GPT were greater(P<0.05) when sheep fed B, C and D compared with A diet,there were no difference(P>0.05) after 4 hours when sheep fed C and D diet compared with A diet.(7)The BUN were increased significantly(P<0.05) after 4 hours when sheep fed B^ C and D compared with Adiet, BUN peak appears after 2 hours.BUN changes with time,there were no difference(P>0.05) among A, B, C and D diets.but BUN were increased by 9% ~ 36.4% respectively when sheep fed B, C and D diets compared with A diet.(8)Blood pH were increased significantly(P<0.05) after 2 hours when sheep fed B diet compared with other diets,there were no difference(P>0.05) when sheep fed other diets,there were no difference(P>0.05) after 4 hours when sheep fed B, C and D diets compared with A diet.The result indicated that it was safety and significantly when sheep fed B, C and D diets. Furthermore, blood biochemical materials were in range of normal physiology, C, D diets has increased the N utilization comparing with A, B diets.更多还原