大鼠肝郁脾虚证胃肠运动功能障碍动物模型的实验研究

【作者】 彭延娟；

【导师】 彭成；

【作者基本信息】 成都中医药大学 ， 方剂学， 2004， 硕士

【摘要】 实验目的：创制大鼠肝郁脾虚证胃肠运动功能障碍动物模型，建立大鼠肝郁脾虚证胃肠运动功能障碍动物模型的生物学特征。 实验方法：应用限制活动加食醋灌胃法建立大鼠肝郁脾虚证胃肠运动功能障碍动物模型，用定量、半定量方法观察肝郁脾虚证的症状表现；用水迷宫法测定大鼠的智力；用HE染色法观察胃肠组织形态学变化；用电镜观察胃肠超微结构变化；用半固体营养糊法测定大鼠的胃和小肠运动功能变化；用多导生理记录仪记录大鼠胃肠的电生理变化；用放免法测定下丘脑及空肠单胺类神经递质5-羟色胺(5-HT)、血浆胃动素(MTL)、胃泌素(Gas)、生长抑素(SS)、血管活性肠肽(VIP)、神经肽Y(NPY)、神经降压素(NT)。 实验结果：①大鼠肝郁脾虚证胃肠运动功能障碍模型的建立：模型组大鼠一般状态较差，被毛蓬松、枯萎，叫声尖细，贴边，喜好扎堆，反应迟缓，抓取时挣扎无力，啃咬铁桶声由强变弱，24小时摄食量、自发活动、体重均减少，腹围指数变大，大便稀软，甚至溏泻，肛周污秽；模型组大鼠胃残留率增加、小肠推进率减慢，与空白组对照有显著性差异(P＜0.05)；②本模型的生物学特征：下丘脑和空肠中的5-HT均升高，与空白对照组有显著性差异(P＜0.05，P＜0.01)；造模后大鼠胃电振幅和胃肠肌条张力均明显降低，与空白组对照有显著性差异(P＜0.05)；血浆胃肠激素水平紊乱：其中VIP、NPY均升高(P＜0.05，P＜0.01)，MTL降低(P＜0.01)，SS有升高的趋势，但无统计学意义(P＞0.05)，NT、Gas均有降低的趋势，亦无统计学意义(P＞0.05)；胃肠组织与胃肠细胞超微结构的病理损害明显。 实验结论：应用限制活动加食醋灌胃法可复制出稳定的肝郁脾虚证胃肠运动障碍动物模型。本模型的生物学特征是：模型大鼠的下丘脑及空肠的5-HT升高，胃电的振幅及胃肠肌条的张力减弱，血浆胃肠激素水平紊乱，胃肠组织细胞的病理变化明显。更多还原

【Abstract】 Objective: To establish the rats model of Liver-Qi depression and Spleen-Qi deficiency Syndrome with the gastroenteric motional function imbalance and its biological characteristics.Methods: This animal model is made by the method of confining the rats’ movement and giving them vinegar by mouth. The animal model’s syndrome is observed with quantitative and half-quantitative method. The animal model’s intelligence level is measured according to water maze. The morphologic change of stomachs small intestine is watched according to HE staining. The ultrastructure change in the stomach and small intestine is observed with electron microscope. The gastroenteric motional function is measured according to the movement of semisolid substance. The lower thalamencephalon and jejunum level of 5-hydroxytryptamine (5-HT) and the plasma level of motilin(MTL) gastrin(Gas) neurotensin(NT) somatastin(SS) vasoactive intestinal polypeptide(VIP) and neuropeptide Y(NPY) are measured by radioimmunoassay(RIA). The electrophysiological change in the stomach and small intestine is noted by the multipurpose recorder.Result: (1)The rats model of Liver-Qi depression and Spleen-Qi deficiency Syndrome with the gastroenteric motional function imbalance have been established. Compared with the controlled rats, the general condition of the model rats is abnormal. Their symptoms are as followings: the puffed hair, the raspy voice with crouching together in the corner, the slow response with no struggle when seized, the decreasing appetite in 24 hours, the reducing movement, the falling body weight, flatulence with the loosen stool and dirty anus. The solid which is left in the stomach increases unusually (P < 0.05 ) . The small intestine’s drive rate becomes slower than that of the controlled rats(P < 0.05 ).(2)The model rats have a series of biological characteristics.In comparison with the controlled group, the lower thalamencephalon and jejunum level of 5-HT increases (P < 0.05, P < 0.01) .The muscular tension of the stomach and small intestine decreases (P < 0.05). The gastroenteric hormone level is abnormal. The plasma level of VIP and NPY increases (P < 0.05,P < 0.01) and the SS increases also. The plasma has lower MTL level (P < 0.01). The plasma level of NT and Gas is lower also. The pathological and ultrastructure change in stomach and small intestine is obvious.Conclusions: Using the above mentioned method, we can establish the stable rat model of liver-Qi depression and Spleen-Qi deficiency Syndrome with the gastroenteric function imbalance. We also set up its biological characteristics. The lower thalamencephalon and jejunum levels of 5-HT increase. The muscular tension of the stomach and small intestine decreases. The gastroenteric hormone level is abnormal. The pathological and ultrastructure change in stomach, small intestine is obvious.更多还原