【作者】 唐权亚；

【导师】 李明权；

【作者基本信息】 成都中医药大学 ， 中西医结合临床， 2004， 硕士

【摘要】 目的：观察中药复方制剂对阿霉素肾病大鼠蛋白尿和电荷屏障的作用，探讨中药复方对肾病综合征的治疗作用及机理。 方法：将SD大鼠随机分为正常组和造模组，将造模组采用尾静脉内一次性注射阿霉素溶液(剂量5.5 mg／kg)，造模后第5天将造模组随机分为模型组、中药复方1组、中药复方2组、中药复方3组、强的松组。造模后第6天开始给药，连续给药24天。测大鼠24h尿蛋白、血清白蛋白、胆固醇、尿素氮、肌酐，并取大鼠肾脏做PEI染色和Alcian蓝(AB)染色，了解其电荷屏障的改变，探讨中药复方降低尿蛋白，治疗NS的机理。 结果：(1)阿霉素模型大鼠于造模后第10天时出现明显蛋白尿。(2)各中药复方和强的松都能明显降低AN大鼠的24h尿蛋白量，各中药组的起效较强的松慢，中药复方2的作用优于中药复方1和3。(2)各中药复方和强的松能升高AN大鼠的血清白蛋白，降低血清胆固醇、肌酐和尿素氮，其中中药复方2和强的松的作用更明显。(3)各治疗大鼠PEI染色和AB染色结果均优于模型组。 结论：三个中药复方和强的松都能降低AN大鼠的24h尿蛋白量，升高血清白蛋白，降低胆固醇、肌酐和尿素氮，其中中药复方2和强的松的作用更明显。治疗各组都能减少肾小球阴电荷丢失，保护肾小球电荷屏障。中药复方对电荷屏障的保护可能是其降低尿蛋白的机制之一。更多还原

【Abstract】 Objective: To investigation effects and mechanisms of compound Chinese formulas on the proteinuria and charge barrier of Adriamycin nephropathy rats which manifest the nephritic syndrome. Methods: Divided the Sprague-Dawley rats into control and normal groups. The Adriamycin nephropathy model rats were established by single transvenous injection of Adriamycin(dosage 5.5 mg/kg) on the control group and the rats were subdivided into model, compound Chinese formulal, compound Chinese formula2, compound Chinese formula3 and prednisone group five days later. Medication was exerted continuously for twenty -four days from the sixth day. Amount of urine protein whthin 24 hours, serum albumin,cholesterol,urea nitrogen and creatinine were examined and renal specimens were obtained.Anionic sites and polyanion were stained with polyethyleimine(PEI) and alcian blue as a cationic probes to know the charge barrier. Effects and mechanisms of compound Chinese formulas were investigate by those ways.Result: (1)Remarkable proteinuria happened around the tenth-day incontrol group caused by adriamycin.(2)Although prednisone did work earlier than compound Chinese formulas, all the compound Chineseformulas had the function to decrease amount of urine protein whthin 24 hours especially the compound Chinese formula2.(3) All compound Chinese formula and prednisone could increase serum albumin, reduce cholesterol,BUN and Scr.(4)The PEI and AB staining of all the groups given meditation showed better results.Conclusion: Compound Chinese formulas and prednisone could decrease the amount of urine protein whthin 24 hours;increase serum albumin; reduce cholesterol ,BUN and Scr especially the compound Chinese formula2 and prednisone. All the groups given meditation could lower the loss of anionic charge. To protect the charge barrier is likely to be one of mechanism of this effect.更多还原