【作者】 王永；

【导师】 杨保收； 李培锋；

【作者基本信息】 内蒙古农业大学 ， 临床兽医学， 2004， 硕士

【摘要】 苦豆子(Sophora alopecuroides L.)系豆科槐属植物，其根、根茎、种子和全草中含有多种生物碱成分，具有特殊的药理学活性，在抗炎、抗微生物、抗肿瘤、免疫等多方面具有独特的作用。目前研究最多的是苦参碱(Matrine)和氧化苦参碱(Oxymatrine),并已开始在医学临床应用。槐果碱（Sophocarpine）是苦豆子生物碱中的一种生物碱单体，本研究采用有机溶剂回流提取法从苦豆子总碱中分离出槐果碱单体，并以丙酮多次重结晶来纯化，经薄层层析鉴定，证明其含单一的槐果碱，纯度较高。采用酸性染料比色法测定槐果碱的含量、标准曲线、回收率及其稳定性，结果证明该方法灵敏度高、重现性好、结果准确。采用简易法测定脂溶性苦豆子总碱和槐果碱对小白鼠的灌胃半数致死量，结果证明脂溶性苦豆子总碱和槐果碱属于中等毒性生物碱。采用足跖致炎法、皮内染料渗出法、耳廓致炎法和棉球肉芽肿法进行了槐果碱抗炎作用研究，结果证明槐果碱对急性、亚急性和慢性增生性炎症均有显著的抑制作用。采用灌胃给药的方法研究槐果碱对小白鼠自然感染的体内、外寄生虫的作用，结果证明槐果碱对小白鼠肠道寄生的线虫有明显的驱杀作用，但对体表寄生的螨虫无显著效果。更多还原

【Abstract】 Sophora alopecuroides L. is a kind of plant, which has many kinds of alkaloids in the seeds and other parts of body. These alkaloids have various effects in pharmacology such as against inflammation, cancer, virus, and parasite.In these years, many studies were done on matrine and oxymatrine, and some active ingredients of them have been used in the clinic. Sophocarpine is a member of total alkaloids in Sophora alopecuroides L.. The objective of the experiment is to separate sophocarpine out from total alkaloids of Sophora alopecuroides L, and identify it by TLC. With acid dye spectrophotometry, the concentration of sophocarpine was measured, the recovery of this method was 98.7%. Several models of inducing inflammation were used in mice to study the anti-inflammatory effect of Sophocarpine. The result showed that Sophocarpine inhibited all these kinds of inflammation in the models significantly. The Sophocarpine was administered to mice infected with parasites by oral routine, the result showed that Sophocarpine had significant anti-parasite effect for nematodes in mice, but had no effect for tetranychus in mice.更多还原