【作者】 吴维明；

【导师】 陈裕泉；

【作者基本信息】 浙江大学 ， 生物医学工程， 2004， 硕士

【摘要】 聚吡咯作为一种导电高分子在传感器中有着广泛的应用。论文对基于丝网印刷电极的聚吡咯气敏传感器的气敏特性和聚吡咯在固定葡萄糖氧化酶制备葡萄糖氧化酶电极中做了初步的研究和探讨。 当某些气体分子吸附于聚吡咯膜时，膜的导电性就会得到改变，通过测量膜的电阻变化就可以得到气体的浓度。实验采用化学气相沉积的方式在丝网印刷电极上沉积聚吡咯气敏膜用于气体的检测，并研究了电化学去掺杂—掺杂对传感器气敏特性的影响。传感器对NH₃响应灵敏，对浓度为10ppm的NH₃都有很好的响应。通过电化学去掺杂后，传感器的灵敏度下降很多，再经电化学掺杂其他离子后，传感器的灵敏度得到提高，其中掺杂ClO₄^-后提高较大。 当聚吡咯在电极上氧化聚合时，会形成带正电荷的聚吡咯骨架，带负电荷的酶就会通过电荷吸引掺杂到骨架中，从而得到固定。实验对聚吡咯／葡萄糖氧化酶电极的制备条件进行了一些研究。实验发现聚吡咯溶液中吡咯单体的浓度、酶的浓度、聚合溶液的pH值、聚合电量等对电极的响应影响较大，当酶的浓度为2mg／ml，吡咯单体浓度为0.05M，LiClO₄的浓度为0.05M，聚合溶液的pH为5.0，聚合电位为0.8V（vs.SCE），聚合电量为2.5mC时，制备的酶电极的响应最大。更多还原

【Abstract】 As one of the famous conducting macromolecules, Polypyrrole is applied extensively in sensors. In this paper, the conditions of the preparation of Polypyrrole/Glucose Oxidase electrode and the characteristic of the Polypyrrole gas sensor was discussed.When some gas molecule is adsorbed in the Polypyrrole membrane, the conductivity of the membrane changes. The concentration of the gas could be detected by measuring the change of the conductivity . In the experiments, the Polypyrrole gas sensor was prepared on screen-printed electrodes by CVD(Chemical Vapor Deposition) . The effect of the electrochemistry undoping and doping on the characteristic of the gas sensor was studied. The sensor was sensitive to NH3, whose concentration was as low as 10ppm. When the Polypyrrole was undopped by electrochemical method, the sensitivity of the sensor reduced. When the Polypyrrole was dopped by electrochemical method again, the sensitivity of the sensor enhanced. The dopping of ClO4- had a great effect on the sensitivity of the sensor.The Glucose Oxidase could be immobilized in the Polypyrrole membrane when the pyrrole is polymerizing on the electrode. In the experiments , the conditions of the preparation of the Polypyrrole/Glucose Oxidase electrode were studied. The concentration of pyrrole, the concentration of the enzyme, the pH of solution, and the volume of charge had a great effect on the preparation of the electrode.When the concentration of enzyme was 2mg/ml,the concentration of pyrrole was 0.05M, the concentration of LiClO4 was 0.05M, the pH of solution was 5.0, the polymerization potential was 0.8V(vs.SCE)and the volume of polymerization charge was 2.5mC, the response of Polypyrrole/Glucose Oxidase electrode was high.更多还原