5-氨基乙酰丙酸诱导原卟啉Ⅸ定量检测及大肠癌荧光光谱研究

【作者】 张波；

【导师】 张阳德；

【作者基本信息】 中南大学 ， 生物医学工程， 2004， 硕士

【摘要】 目的：利用5-ALA诱导产生的内源性PpIX在大肠癌组织细胞内积聚的特性，定量分析了大肠正常组织和不同时期癌组织中的PpIX含量，以及正常鼠、肿瘤诱导鼠及其第二代的血液PpIX含量。 材料和方法：20只DMH诱导的大肠癌模型SD大鼠，12只大肠癌模型SD大鼠的第二代，8只正常SD大鼠，按25mg／kg．wt尾静脉注射5-ALA，150分钟后采取血液和组织标本。用化学抽提法提取血液和组织PpIX。设置激发波长403nm，狭缝10nm；发射波长400～700nm，狭缝20nm；扫描速度240nm／分，用Hitachi-4500荧光分光光度计测其荧光强度，计算PpIX含量。 结果：在正常组、早癌组和进展期癌中，血液和组织的PpIX含量均具有统计学意义差别，其血液与组织PpIX含量的平均值分别为2363.44±241.25ng／ml，3357.39±369.11ng／ml，4939.55±692.68ng／ml；622.97±61.85ng／g，763.098±83.93ng／g，844.16±87.56ng／g。 结论：静脉注射25mg／kg．wt的5-ALA，可以使PpIX在正常鼠和肿瘤鼠血液，正常组织和肿瘤组织内的积聚具有明显差异性。更多还原

【Abstract】 Objective: The selective accumulation of endogenous PpIX in rats colon tissue following systemic administration of 5-ALA with the dose of 25mg/kg wt and interval time of 150min has been examined. The correlation of the PpIX concentration distribution in blood and tissue with pathological change has been studied.Materials and Methods: Multifocal colon cancers were induced by 25mg/kg.wt injections of 1,2-DMH in SD rats, the second era of induced rats and normal SD rats. Chemical extraction assays of the PpIX content in tissue and blood were carried out at 150 min after trail intravenous injections of 5-ALA at a dose of 25mg/kg.wt with fluorescence spectrophotometer.Results: A significant difference of PpIX content was found in tissue and blood, with a mean of 2363.44 +241.25ng/ml, 3357.39 + 369.11 ng/ml, 4939.55 + 692.68 ng/ml in blood and 622.97 + 61.85 ng/g, 763.098 + 83.93 ng/g , 844.16 + 87.56ng/g in tissue respectively corresponding to advanced cancer, early cancer and normal rats.Conclusions: Systemic administration of low dose 5-ALA can induce effective tissue sensitization with PpIX and appears to be a promising technique for PDD.更多还原