【作者】 龙宇红；

【导师】 李向荣；

【作者基本信息】 浙江大学 ， 营养与食品卫生， 2004， 硕士

【摘要】 目的：探讨维生素C、维生素E对染铅大鼠血液系统和海马组织的保护作用及机理。 方法： 1 维生素C、维生素E对实验性铅中毒大鼠血液系统的预防作用 SD健康雄性大鼠25只，随机分为5组，每组5只。空白组大鼠自由饮食和饮用去离子水五周；铅模型组大鼠自由饮用0.02％的醋酸铅(去离子水配制而成)四周，第五周自由饮用去离子水；预防组大鼠在染铅同时以维生素C 100mg／kg.bw、维生素E 100mg／kg.bw剂量单独或联合灌胃。实验第四周时测量血中铁、锌、钙、铅、Hb和δ-ALAD浓度。 2 维生素C、维生素E对实验性铅中毒大鼠血液系统的治疗作用 SD健康雄性大鼠25只，随机分为5组，每组5只。空白组和铅模型组大鼠处理同前，治疗组大鼠染铅四周后以维生素C 100mg／kg.bw、维生素E 100mg／kg.bw剂量单独或联合灌胃一周。测量四、五周时血中铁、锌、钙、铅、Hb和δ-ALAD浓度。 3 维生素C、维生素E对实验性铅中毒大鼠海马组织的保护作用 各组大鼠处理同实验方法2，实验第五周，颈椎脱臼处死大鼠，股动脉放血，取双侧海马组织，制备10％的组织匀浆，测量MDA、SOD、GPx、NOS、NO。 结果： 1 维生素C、维生素E对实验性铅中毒大鼠血液系统的预防作用沁介砍掌硕士学位论文 预防组与铅模型组第四周血样指标相比较，血铅浓度降低，且呈显著性差异(P< 0.05);血铁浓度、Hb和6一ALAD浓度与铅模型组相比，有显著性差异(P<0.05)。钙、锌浓度与铅模型组相比，无显著性差异 (P>0 .05)。 2维生素C、维生素E对实验性铅中毒大鼠血液系统的治疗作用 治疗组治疗后血铅浓度低于铅模型组，且有显著差异性(P<0.05);血铁、Hb、6一ALAD治疗后与模型组数据显示，治疗后的浓度均高于铅模型组，呈显著性差异(P<0 .05);血钙、血锌浓度治疗前后相比，无显著性差异(P>0.05);与模型组相比，无显著性差异(P>0.05)。 3维生素C、维生素E对实验性铅中毒大鼠海马保护作用 对于MDA，治疗组显著高于铅模型组，且其联合治疗组与vitC单独治疗组呈显著性差异(P<0.肠);对于s0D，治疗组显著高于铅模型组(P<0.05);对于GPx、NO、NOs，治疗组高于铅模型组，呈显著性差异(P<0 .05)。结论:在铅中毒的同时及其后，补充维生素C、维生素E可降低血铅，升高血铁浓度，恢复ALAD和Hb的活性，改善铅导致的血液系统损伤。治疗性补充维生素C、维生素E可提高海马的抗脂质过氧化能力，逆转NOS，N0活性，保护海马免受铅损伤。更多还原

【Abstract】 Objective: To study the effect and mechanism of VitC, VitE on the blood system and hippocampus of lead poisoning SD rats. Methods:Preventive effect of Vitmin C, Vitamin E on blood system of lead exposed SD rats in development phase25 male SD rats were divided randomly into 5 groups. Blank group, eat and drink freely, lead control group, drink 0. 02% lead acetate (mixed by deionized water) for four weeks. Preventive groups, fed by vitamin C 100mg/kg. bw, vitamin E 100mg/kg. bw, or vitamin C (100mg/kg. bw ) and vitamin E (100mg/kg. bw)respectively at the same time of drinking 0.02% lead acetate. At week 4, concentration of Fe, Zn, Ca, Pb, Hb and -ALAD in blood were measured.Therapeutical effect of Vitmin C, Vitamin E on lead exposed SD rats in development phase25 male SD rats were divided randomly into 5 groups, therapy groups, fed by vitamin C 100mg/kg. bw, vitamin E lOOmg/kg. bw, or vitamin C and vitamin E respectively for one week, after drinking 0. 02% lead acetate for 4 weeks. At week 5, all rats are sacrificed by luxation of cervical vertebra, concentration of Fe, Zn, Ca, Pb, Hb and 8 -ALAD in blood, and activity of SOD, GPx, MDA, NOS and NO of hippocampus were measured. Results: Preventive effect of Vitmin C, Vitamin E on blood systemBlood -ALAD, Kb, Zinc concentration of preventive groups increased significantly than that of lead control group (p<0. 05), but concentration of Pb was lower than lead control gfoup(P<0.05) .serum calciunu zinc level was no significance between these groups (p>0.05).Therapeutical effect of Vitmin C^ Vitamin E on blood system At week 5, concentration of Pb in therapy groups were lower than that of lead control group significantly (p<0. 05). Concentration of Fe, Hb, 8 -ALAD were higher than lead control group significantly(p<0.05). Between all therapy groups and lead control group, no significance was observed about concentration of Ca, Zn (p>0. 05).Protection of Vitamin C, Vitamin E on hippocampus As MDA, its concentration of therapy groups were higher than lead control group significantly(p<0. 05), and among therapy groups, concentration of MDA in combination of VitC and VitE group was higher than VitC therapy groups significantly(p<0.05). As SOD, GPx, NO, NOS, their concentration were higher than lead control group significantly(p<0. 05). Conclusion:Supplied Prophylactically or treat with Vitamin E^ Vitamin C can low blood lead level, increase Fe level, recover activity of ALAD and Hb, decrease injury of blood system by lead. In addition, VitC and VitE can protect hippocampus from Lipid peroxidation, and inversio NO, NOS in lead poisoing rats.更多还原