【作者】 李香芬；

【导师】 李沛；

【作者基本信息】 福建中医学院 ， 针灸推拿， 2003， 硕士

【摘要】 目的：探讨针刺对正常健康育龄女性生殖内分泌的影响。 方法：从60位22-35岁的女性自愿者中筛选出28位月经周期规则，基础体温呈现典型排卵特征的作为观察对象，采用自身对照法，针刺体穴“石门”、“三阴交”（单侧），耳穴“G₄区”（双侧），观察针刺对外周血中，（1）垂体促性腺激素：促卵泡激素（FSH）、促黄体生长激素（LH）；（2）卵巢性激素：雌二醇（EA-2）、睾酮（T）、孕酮（P）等水平的影响。 结果：针刺对垂体促性腺激素（FSH）、（LH）的分泌无明显影响，对卵巢性激素（E₂、T、P）的分泌具有明显的抑制性效应，其作用机理，有待进一步观察。 结论：针刺对女性生殖内分泌具有一定的调节作用。 前期动物实验研究表明：实验1：针刺对雌性恒河猴月经周期外周血中FSH、LH、E₂、T的分泌水平的影响。结果表明针刺对生殖内分泌有明显的抑制效应，尤其是对各激素分泌峰波的形成影响更为明显，揭示了对正常排卵过程的可能干扰。实验2：针刺不同穴位对雌性恒河猴月经周期外周血中FSH、LH、E₂、T分泌水平的影响。结果表明，单用体穴或单用耳穴都能对雌性恒河猴月经周期卵泡期外周血中FSH、LH、E₂、T的分泌产生抑制作用，体穴的作用强于耳穴，同时针刺体穴加耳穴则有一定协同作用。实验3：不同时间针刺对雌性恒河猴生殖内分泌的影响。结果提示：（1）针刺抑制FSH、LH、E₂、T分泌的有效时机在月经周期的卵泡期。（2）针刺抑制FSH、LH、T分泌的最佳时间在早晨，对E₂则在晚上。（3）针刺的抑制性影响的时效性大约24小时。（4）针刺的抑制性作用与对“下丘脑——垂体——卵巢轴”及“下丘脑——垂体——肾上腺轴”的影响有关。更多还原

【Abstract】 Objective: To discuss the effect of acupuncture on genital- endocrine function in normal healthy females during reproductive life.Methods: Among 60 female volunteers at the age of 22-35y, 28 cases with regular menstrual cycle, whose basic body temperature were characterized as typical ovulation, were selected. Self-contrast method was adopted. After acupuncture on unilateral Shimen (KN5), Sanyinjiao (SP6) and bilateral auricular "G4" region, the effect of acupuncture on peripheral serum (1) Pituitary gonadotrophin: follicle stimulating hormone (FSH), Luteinizing hormone (LH); (2) Ovarian sex hormone: estradiol (E2), testosterone (T) and progesterone (P) levels were observed respectively.Results: No significant effect of acupuncture showed on the excretion of Pituitary gonadotrophin (FSH and LH) , but the significant inhibition effect showed on the excretion of ovarian sex hormone ( E2, T and P) . The action mechanism is waiting for further observation.Conclusion: Acupuncture therapy had regulation effect on genital-endocrine function in females. The experimental study in animals at early days indicated as follows:Experiment 1: The effect of acupuncture therapy on serum FSH, LH, E2 and T levels in female rhesus during menstrual cycle. Acupuncture on Shimen (RN5), Sanyinjiao (SP6) and auricular "G4 " region showed apparent inhibition effect, especially on the formation of peak wave of the hormones, demonstrating the interference effect on normal ovulation procedure.Experiment 2: The effect of acupuncture therapy at different acupoints in female rhesus. The result indicated that either body points or auricular points had inhibitory effect on the excretion of serum FSH, LH, E2 and T, but the effect of body points was superior to that of auricular points. The effect was strengthened when body points were selected combined withauricular points, demonstrating the relative specificity of acupoints. And the therapeutic effect of acupoints was enhanced.Experiment 3: The effect of acupuncture therapy in different time on genital- endocrine (unction in female rhesus. The result showed as follows: (1) The effective opportunity of inhibition by acupuncture on the excretion of FSH, LH,E2 and Tought to be during follicular phase of menstrual cycle. (2) The best time of inhibitory effect by acupuncture on the excretion of FSH, LH and T ought to be in the morning, but the effect on E2 ought to be in the evening. (3) The effective period of inhibitory effect by acupuncture was about 24 hours. (4) The inhibitory effect of acupuncture was related to the function of hypothalamic-pituitary-ovary axis and adrenal cortex. The study demonstrated the best manipulation time of acupuncture and the new application on study methods of traditional birth control.更多还原