中国林蛙卵活性部位的化学成分研究

【作者】 卢立明；

【导师】 徐绥绪；

【作者基本信息】 沈阳药科大学 ， 药物化学， 2002， 硕士

【摘要】 本文以中国林蛙(Ranatemporaria chensinesis David)的卵为原料，对其活性部位二氧化碳超临界萃取物化学成分进行了初步研究。 利用硅胶柱色谱，制备硅胶薄层色谱，葡聚糖凝胶柱色谱，制备高效液相色谱等分离手段，从中国林蛙卵活性部位中分离得到15个单体和一个混合物，通过理化常数测定，光谱数据分析及化学方法等，鉴定了其中13个化合物的结构，分别为胆固醇十六烷酸酯(cholesterol, palmitote)(Ⅰ)，十六烷酸乙酯(hexadecanoic acid, ethyl ester)和十八烷酸乙酯(octadecanoic acid, ethyl ester)混合物(Ⅱ)和(Ⅲ)，硬脂酸(octadecanoic acid)(Ⅳ)，胆固醇(cholesterol)(Ⅴ)，棕榈酸-α，α′-双甘油酯(hexadecanoic acid, 2-hydroxy-1, 3-propanediylester)(Ⅵ)，棕榈酸(palmitic acid)(Ⅶ)，胆甾-5-烯·7-酮(cholest-5-en-7-one, 3-hydroxy)(Ⅷ)，棕榈酰胺(hexadecanamide)(Ⅸ)，油酸-α-单甘油酯(9-octadecenoic acid, 2, 3-dihydroxypropyl ester)(Ⅹ)，棕榈酸-α-单甘油酯(hexadecanoic acid, 2,3-dihydroxypropyl ester)(Ⅺ)，壬二酸(azelaic acid)(Ⅻ)，3，5，6-三羟基-胆甾烷(cholestane-3β，5α，6β-triol)(ⅩⅢ)。以上化合物均为首次从中国林蛙卵中分离得到。此外，利用气相色谱-质谱(GC-MS)联用技术对中国林蛙卵中部分脂肪酸成分进行了初步定性分析，共鉴定21种脂肪酸。 药理活性研究显示，中国林蛙卵及其化学成分具有明显的抗士的宁惊厥活性。更多还原

【Abstract】 In this thesis, the chemical constituents and activities of the ovum of Rana temporaria chensinensis David were studied.15 compounds were isolated from the ovum of Rana temporaria chensinensis David with silica gel, sephadex LH-20, HPLC etc. On the basis of analysis of UV, IR, NMR, MS data and measuring of physico-chemical characters, the structures of 13 compounds were elucidated as cholesterol, palmitote (I), hexadecanoic acid, ethyl ester and octadecanoic acid, ethyl ester (II) and (III), octadecanoic acid (IV), cholesterol (V), hexadecanoic acid, 2-hydroxy-1, 3-propanediyl ester (VI) , palmitic acid (VII), cholest-5-en-7-one, 3-hydroxy (VIII), hexadecanamide (IX), 9-octadecenoic acid, 2, 3-dihydroxypropyl ester (X), hexadecanoic acid, 2, 3-dihydroxypropyl ester (XI), azelaicacid (XII), 3, 5, 6-cholestane-3, 5a,6-triol (XIII), all these compounds were isolated for the first time from the ovum of Rana temporaria chensinensis David. Furthermore, 21 kinds of fatty acids were identified by GC-MS.Studies on activities of the chemical constituents from the ovum of Rana temporaria chensinensis David showed potent anti-convulsion effects in convulsion mice induced by strychnine nitrate in single dose.更多还原