虎杖的化学成分及其质量研究

【作者】 俸灵林；

【导师】 包文芳；

【作者基本信息】 沈阳药科大学 ， 生药学， 2003， 硕士

【摘要】 虎杖为蓼科蓼属多年生草本植物虎杖(Polygonum cuspidatum Sieb.et Zucc.)的根及根茎，是一种常用中药。植物资源丰富，分布于全国各地。 本文对虎杖的根及根茎进行了化学成分的研究，从中分离得到了17个化合物，通过理化常数和波谱数据分析等方法鉴定了其中的14个，分别为大黄酚(chrysophanol，1)、大黄素-6-甲醚(physcion，2)、大黄素(emodin，3)、大黄酸(rhein，4)、白藜芦醇(reveratrol，5)、白藜芦醇苷(piceid，6)、芹菜素(apigenin，7)、山奈酚(kaempferol，8)、槲皮素(quercetin，9)、β-谷甾醇(β-sitosterol，10)、豆甾-5-烯-3β，7α-二醇(stigmast-5-ene-3β，7α-diol，11)、胡萝卜苷(daucosterol，12)、没食子酸(gallic acid，13)和D-甘露醇(D-mannitol，14)。其中化合物11为首次从本属植物中获得的已知化合物。 依据近期对虎杖药理作用研究的热点和新趋势，确定了能代表虎杖对心血管系统及抗肿瘤作用的白藜芦醇和白藜芦醇苷为虎杖质量分析的指标性成分，并建立了国内首次用高效液相色谱法同时测定虎杖中白藜芦醇和白藜芦醇苷的分析方法。此外，还详细考察了白藜芦醇和白藜芦醇苷的稳定性。 首次对不同产地的虎杖药材、不同采收期的虎杖根及根茎以及虎杖的不同部位中的白藜芦醇和白藜芦醇苷进行了含量测定，对虎杖内在质量进行了较为全面的研究。 本研究为虎杖的化学成分及质量的进一步研究提供了可靠的实验依据和方法。更多还原

【Abstract】 Polygonum cuspidatum Sieb. et Zucc. is one of traditional Chinese medicines in common use with its radix and rhizome. It is a member of Polygonum Linn.. Abundant in natural resources, this species has wide distribution in China.In this dissertation, studies on chemical constituents of the radix and rhizome of Polygonum cuspidatum are presented firstly. 17 compounds were isolated from the extraction of radix and rhizome. Among them, 14 compounds’ structures were identified on the ground of physical, chemical properties and spectral data. The 14 compounds respectively are chrysophanol(1), physcion(2), emodin(3), rhein(4), reveratrol(5), piceid(6), apigenin(7), kaempferol(8), quercetin(9), β-sitosterol(10), stigmast-5-ene-3β ,7α -diol(11), daucosterol(12) , gallic acid(13) and D-mannitol(14). stigmast-5-ene-3β ,7 -diol (11) is isolated from the genus for the first time.Recently, resveratrol and piceid, aboundant in Polygonum cuspidatum, are found that they have specific pharmaceutical activities such as natable cancer chemopreventive activity, reducing the risk of coronary heart disease (CHD) and ect. Therefore, according to the new research tendency of Polygonum cuspidatum’s pharmacologinical effects, we determined resveratrol and piceid as the constituents for quality evaluation of Polygonum cuspidatum, which can nearly represent the effect of Polygonum cuspidatum, .The method of simultaneous determination of resveratrol and piceid in Polygonum cuspidatum was developed for the analysis of the crude drug’s quanlity by using HPLC. It is the frist time in China to establish such a method. Besides, the stability ofresveratrol and piceid was examined carefully.Moreover, the resveratrol and piceid of 25 samples were determined by the above method. The samples included the radix and rhizome of Polygonum cuspidatum of different habitats, different collecting times and different parts of this plant. It also is the first time to study the inherence quanlity of Polygonum cuspidatum relatively completely up to now.These afford reliable experimental basis for the chemistry and quanlity research on Polygonum cuspidatum in the future.更多还原