【作者】 孙丰梅；

【导师】 刘安军； 张志胜；

【作者基本信息】 河北农业大学 ， 动物营养与饲料科学， 2003， 硕士

【摘要】 硫酸软骨素是来源于动物软骨组织的一类重要的酸性粘多糖。本试验以羊的软骨为原料，选择最佳的生产工艺来提取，并以小白鼠为试验动物，研究其降血脂和抗氧化的保健功能。 试验一：以羊的气管为原料，采用稀碱-浓盐工艺法提取，确定最佳的工艺条件。结果表明：用20％的NaCl浓盐浸提，期间用NaOH不断调节pH值。得出，在40℃提取，90℃盐解，酒精浓度为70％时，产品质量合格。将第一次沉淀物重新溶解后再沉淀，解决了澄清度不合格的问题。最后应用本方法，从羊的软骨中提取CS的平均收率是7.6％，含量为84.9％，澄清度0.018，氮含量2.97％，粉末洁白。 试验二：选择体重、年龄相近的100只SR系雄性小白鼠作试验动物，随机分为5组，每组20只。其中，Ⅰ组饲喂高脂饲料，Ⅱ、Ⅲ、Ⅳ组在高脂饲料的基础上，分别添加0.5g／kg、1.0g／kg、1.5g／kg的硫酸软骨素，Ⅴ组饲喂正常饲料，研究其降血脂和抗氧化的功能。结果表明：(1)CS在高脂饲料中添加后，对高血脂小鼠的血脂指标影响不一，添加量为0.5g／kg时，各项指标与对照高脂组相比，差异不显著(P＞0.05)；添加量为1.0g／kg时，能够极显著地降低血清总胆固醇(TC)的水平(P＜0.01)，对甘油三酯(TG)的影响只达到显著水平(P＜0.05)，对高密度脂蛋白胆固醇(HDL-C)的影响还是不显著(P＞0.05)，对低密度脂蛋白胆固醇(LDL-C)的降低达到显著水平(P＜0.05)；添加量为1.5g／kg时，TC与对照高脂组相比，差异极显著(P＜0.01)，对TG和LDL-C的影响均为极显著(P＜0.01)，而对HDL-C的作用达到显著水平(P＜0.05)。(2)：CS对机体抗氧化性能的影响中，当添加量为0.5g／k9时，各项指标与对照组相比，均不明显(P＞0.05)；当添加量为1.0g／kg时，对谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)的影响达到显著水平(P＜0.05)，但对超氧化物歧化酶(SOD)的作用还是不明显(P＞0.05)；添加量为1.5g／kg时，对GSH-PX的活性和MDA的影响达到极显著水平(P＜0.01)，而对SOD的影响则达到显著水平(P＜0.05)。更多还原

【Abstract】 Chondroitin Sulfate(CS) was one of the important mucopolysaccharides from animal cartilage tissues. This thesis was to choose the optimum extracting conditions of CS from cartilage and study its effect on reducing blood-lipid, anti-oxidation in small rats.In experiment 1, CS was extracted from trachea of sheep by using dilute alkali-dense salt method and the best extraction conditions confirmed. The results showed as follows: During the CS was extracted by 20%NaCl, the liquid pH value was continuously regulated by NaOH. When the extraction temperature, salt extraction temperature, ethyl alcohol precipitation concentration was 40’C, 90*Cand 70% respectively, the quality of product was the highest. The question of defecation was settled by secondly precipitating the solution which was obtained by dissolving the first sediment. Under the best extraction conditions, the white powder of CS can be obtained with the average yield rate of 7.6%, the purity of 84.9%, the defecation degree of 0.018, and the amount of protein of 2.97%.In experiment 2, one hundred small SR rats were selected with near weight and age, and randomly divided into five groups: control group (group I),was reared on a high level fat diet, group II, III-. IV in addition to the high level fat diet of 0.5/kg, 1.0g/kg, 1.5g/kg of CS were given respectively and normal group (group V)was fed on the basal diet . Effect of CS on declining blood lipid and anti-oxidation were studied. The results were followed: (1)In blood lipidparameters, when the level of CS was 0.5g/kg, every index was not significant difference compared with control group (P>0.05);When addition was 1 .0g/kg, TC was decreased very significantly (P<0.01), TG and LDL-C were declined significantly (P<0.05), but had no effect on HDL-C (P>0.05);As for group IV compared with control group, TC ,TG and LDL-C were all strong significantly different (P<0.01), but HDL-C was significantly different (P<0.05) . (2) In anti-oxidation aspect, group II compared with group I , SOD, GSH-PX and MDA were not different respectively (P>0.05); When the addition was 1.0g/kg , GSH-PX was increased and MDA decreased significantly respectively (P<0.05) , but SOD had not rose (P>0.05); For the level of CS was 1.5g/kg , it affected GSH-PX and MDA strong significantly different (P<0.01), while SOD rose significantly (P<0.05).更多还原