【作者】 马道荣；

【导师】 潘丽军；

【作者基本信息】 合肥工业大学 ， 农产品加工及贮藏工程， 2003， 硕士

【摘要】 膨胀床吸附层析技术是分离工程领域近年来研究开发的一种新型分离技术。它将预处理、浓缩和目标产品的捕获集中在一个单元完成，操作类似于固定床，具有返混小、柱效高、操作方便快捷的优点。 茶叶的有效成分茶多酚由十多种儿茶素单体构成，其中大部分属酯型儿茶素。儿茶素具有抗氧化、降血脂、降血压、抗衰变、抗辐射、抗癌防癌等功能，因此在医药、食品工业中有广泛的应用。但由于儿茶素单体性质相近、结构相似、易氧化等原因，其分离纯化难度大、效率低、储藏成本高，规模化生产存在瓶颈。 为了解决上述问题，本课题首次将膨胀床吸附层析技术应用于儿茶素的分离提纯，主要进行了膨胀床系统配置及分流器的设计，大颗粒、低密度的吸附树脂H103、707和XAD-4在床内的膨胀特性和混合特性的研究，装填H103树脂的膨胀床吸附儿茶素的吸附模型研究，膨胀床高效吸附儿茶素的工艺研究，并得出以下结论： 大颗粒、低密度的吸附树脂H103、707和XAD-4在床内的膨胀特性符合Richardson-zaki方程u／u_t=εⁿ； 混合特性可用无因次方程来表达； 流动模型可用一维（轴向）扩散模型来描述； 装填H103树脂的膨胀床吸附儿茶素的吸附等温线用Langmuir方程描述，吸附动力学曲线可用At=ae^b／t方程模拟； 在吸附过程中，采用浓度梯度方式进样可明显提高树脂对儿茶素的吸附量； 膨胀床吸附层析的儿茶素粗品比溶剂萃取法所得产品的品质有所提高。 该研究通过儿茶素的吸附分离对膨胀床技术应用于性质与结构相近的小分子物质的分离方面作了有益的探索，为规模化高效分离儿茶素提供了技术支持。更多还原

【Abstract】 Expanded bed adsorption (EBA) chromatography is becoming a new separation method in bio-separation engineering. During the separation process, concentration and capture of desired product are simplified as one unit operation. Two important features of the expanded bed compared with packed bed are its low back-mixing and high efficiency.Catechin, a family compound of more than 10 monomers, is the main component of Tea Polyphenols (TP), most of which are esters. It has uniquely practical values in many fields, especially in Food industrial and Bio-medical science, such as oxidation resistance, blood pressure declination, radiation resistance and cancer prevention. Due to catechin monomers’ similar characteristics, similar structure and easily oxidized, it is difficult to separate the catechin monomers efficiently and make the cost of reservation higher. This has restricted the productive efficiency of catechin.In order to solve these problems, we applied the expanded bed chromatography to the separation and analysis of crude catechin firstly. The main contents are listed as follows: The figuration of expanded bed system and the designing of its distributor; The study of expanded-bed characteristics with the resin H103, 707, XAD-4 as adsorbents; The adsorption modeling study of the expanded bed loaded with H103 resin; The high adsorption technology study of expanded-bed. The results are achieved as follows:The expansion characteristic accords with Richardson-Zaki equation; The mixed property is expressed with a math formula; The fluidization model is expressed: The adsorption isotherm can be pictured as Langmuir equation; The adsorption equation can be simulated as At=ae equation; The adsorption yield and quality is improved by adjusting the concentration of tea extracts.Based on the results, it has achieved good effect on separating catechin in expanded bed. This is a good attempt and providing technology on separating small molecular.更多还原