【作者】 雷瓒；

【导师】 黄长江；

【作者基本信息】 暨南大学 ， 环境科学， 2003， 硕士

【摘要】 有机锡化合物对近海生态系统潜在的危害相当严重，但我国还没有制定出相应的法规和标准进行控制，这主要是由于没有便捷、灵敏的研究调查方法为制订相应的法规提供科学依据。 本课题选择利用顶空固相微萃取-气相色谱与火焰光度仪联用(SPME-GC-FPD)技术对海洋环境中的有机锡，特别是三丁基锡(TBT)进行检测。通过一系列实验建立起分析测定水样、底泥及生物样中有机锡含量的灵敏检测方法，并将该方法用于我国东南沿海港口的有机锡污染现状调查。其中，对于泥样和生物样尝试采用了超声处理以及酶解等预处理方法，结果证明能有效提高检测的灵敏度。 对我国东南沿海8个港口的调查结果表明，水样中TBT含量为低于检测限至9.83ng／L，泥样中为低于检测限至174.67ng／g(d.w)，生物样中为2.11-70.60ng／g(w.w)。其中北山湾站点水样中TBT含量低于检测限，生物样中含量最低，但该站点海产腹足类性畸变的调查表明疣荔枝螺已经发生性畸变。因此，我国东南沿海的有机锡污染是普遍和严重的，并已对生态系统构成了威胁。 有机锡污染物分布规律主要由地理环境因素决定，同一港口的港内样品中污染物含量明显高于港外样品。经t-test检验，各调查港口水样和泥样中的TBT和MBT(一丁基锡)含量在港内与港外存在显著差异。而且港口吞吐量与水域环境污染程度也呈显著正相关关系。 疣荔枝螺体内的TBT含量与其性畸变指数VDSI拟合所得S曲线表明，有机锡污染是疣荔枝螺性畸变的诱导因素，其VDSI指数可以作为有机锡污染的生物监测指标。 柘林湾柱状泥样中有机锡化合物垂直分布规律呈单峰型，峰值位于2-3cm深度，6cm以下有机锡化合物含量非常低，这为泥样的采集提供了一定理论依据。更多还原

【Abstract】 Though organotins have caused serious potential damage to the coast ecosystem, there was no corresponding laws, regulations or restrictions to control them in china due to unavailability of sensitive and practical investigating methods.Headspace solid phase microextraction combined with gas chromatography and flame photometric detector was adopted in the present study to determine the organotins, especially tributyltin, in oceanic environment. The sensitive methods for water, sediment and biotic samples were established based on a series of experiments, and then used to survey on occurrence of organotin pollution in harbors along the southeast coast of China. The results showed that the pretreatments of sediment and biotic samples with ultrasonic and enzymic hydrolysis could effectively enhance the sensitivity of determination.The investigated results on 8 harbors along the southeast coast of China showed that the TBT concentrations were from less than detection limit to 9.83 ng/L in water samples, from less than detection limit to 174.67 ng/g(d.w) in sediment samples, and were 2.11-70.61 ng/g(w.w) in biotic samples. The TBT concentration in water sample from Beishan Bay was below detection limit, and was the lowest for biotic sample. However, study on imposex of marine Gastropod showed Thais clavigera in Beishan Bay was imposex. This indicated that occurrence of organotin along the southeast coast of china was universal and serious, and had given damage to the ecosystem.The distribution of organotins in most of harbors was controlled by the geographic facters, such as organotins in all three kinds of samples from the inner harbors were higher than those of the outer harbors. T-test showed significant difference between the two groups of data (inner and outer harbors). Moreover, significant positive correlations were found between organotin concentrations and the throughput capacities of harbors.The S-curve fitting to TBT concentrations and VDSI in Thais clavigera, indicated that the organotin pollution was a key driving force to imposex in Thais clavigera, and the VDSI of Thais clavigera was useful and practical for biomonitoring of organotins pollution.The pattern of vertical distribution of organotin in sediment cores of Zhelin Bay was mono-peak. The peak located in 2-3 cm depth, organotin concentrations were very low below 6 cm depth. This provided an academic fundament for sampling of sediment.更多还原