【作者】 董晔；

【导师】 曾宪贤； 张石峰； 马凤云；

【作者基本信息】 新疆大学 ， 化学工艺， 2003， 硕士

【摘要】 将离子束应用于纤维分解细菌是诱变育种的新尝试。同时，纤维分解细菌的低能离子注入研究，对于更深入地研究离子束生物学效应，拓展离子束生物技术在工业微生物育种的应用范围有一定的意义。 在未提供出发菌原始资料的情况下，对出发菌的斜面培养基及摇瓶配方、生长习性等进行了摸索，确立了基本的发酵条件。 选择了纤维分解细菌酶活测定方法以及DNS法测定还原糖的最适条件，对纤维素分解细菌的生长特性及纤维素酶的最适温度、pH值、温度和pH值的稳定性进行了探索。在此基础上，确定了最佳离子束诱变参数。 离子注入对纤维分解细菌HY2进行诱变育种，效果显著。经筛选得到高产菌株HY2106，其滤纸酶活(FPA酶)和羧甲基纤维素酶活(CMCase酶)分别提高了127.8％和110.2％。同时考察了其遗传稳定性，对比了高产菌HY2106和出发菌HY2对麦草粉中纤维素、半纤维素、木质素的降解能力。更多还原

【Abstract】 Applying the ion beam to the Bacteria of Cellulose hydrolysis is a new attempt. At the same time, the research on the Bacteria of Cellulose hydrolysis implanted by low energy ion will further the study on the biological effect of ion beam and widen the application range of ion beam biotechnology in the industrially microbiological screening.Without documents related to original strain, this thesis had to probe into the primary technological conditions of original strain. And eventually made the certain radial fermentation condition.Determination of cellulase activity and reducing sugar by Dinitrosalicylic (DNS) acid reagent were optimized. The physiological character of cellulase and the best culture pH, temperature and stability were quested for. Then according to this base, this thesis analyzed the influence of some parameters, including energy and dose for the purpose of finding the best ion mutant parameters, and studied the mechanism of implanted ion on this strain.The original strain of the Bacteria of Cellulose hydrolysis HY2 was optimized by using ion implantation and then was induced and screening. At last the high-yielded strain HY2106 was acquired. Its Filter Paper Activity (FPA) enzyme and Carboxymethylcellulose (CMCase) enzyme was respectively enhanced 127.8% and 110.2%, and the genetic stability was detected. The decomposable capability of high-yielded strain HY2106 and original strain HY2 was compared.更多还原